Monocytes from preeclamptic women previously treated with silibinin attenuate oxidative stress in human endothelial cells

被引：1

作者：

Gomes, Virginia Juliani ^{[1
]}

Nunes, Priscila Rezeck ^{[1
]}

Haworth, Sarah McCann ^{[2
]}

Sandrim, Valeria Cristina ^{[3
]}

Peracoli, Jose Carlos ^{[1
]}

Peracoli, Maria Terezinha S. ^{[3
]}

Carlstrom, Mattias ^{[2
]}

机构：

[1] Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618687 Botucatu, SP, Brazil

[2] Karolinska Inst, Dept Physiol & Pharmacol, Stockholm, Sweden

[3] Sao Paulo State Univ Unesp, Inst Biosci Botucatu, Dept Biol & Chem Sci, Botucatu, SP, Brazil

来源：

HYPERTENSION IN PREGNANCY | 2021年 / 40卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

HUVEC; monocytes; oxidative stress; preeclampsia; silibinin; NECROSIS-FACTOR-ALPHA; NITRIC-OXIDE; MECHANISM; PATHWAY;

D O I：

10.1080/10641955.2021.1884258

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate whether the supernatant from monocytes of preeclamptic and normotensive pregnant women, cultured in vitro with silibinin, can modulate oxidative stress in HUVEC. Methods: Concentrations of IL-1 beta, IL-10, and TNF-alpha in monocyte culture supernatants were determined by ELISA. HUVEC and their supernatant cultures were employed for determination of NO, nitrite and nitrate, lipid peroxidation, and hemeoxygenase-1 (HO-1). Results: HUVEC treatment with supernatant of preeclamptic monocytes cultured with silibinin produced increased levels of nitrite, reduced lipid peroxidation, and increased HO-1. Conclusion: Supernatant of monocytes from preeclamptic women induce oxidative stress in HUVEC which can be reduced by silibinin treatment.

引用

页码：124 / 132

页数：9

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