The presence of Prevotella melaninogenica within tissue and preliminary study on its role in the pathogenesis of oral lichen planus

被引:21
作者
Zheng, Sai-wei [1 ]
Xu, Pan [1 ]
Cai, Li-ting [2 ]
Tan, Zheng-wu [1 ]
Guo, Yi-ting [1 ]
Zhu, Rui-xin [2 ]
He, Yuan [1 ]
机构
[1] Tongji Univ, Shanghai Engn Res Ctr Tooth Restorat & Regenerat, Sch Stomatol, 399 Middle Yanchang Rd, Shanghai 200072, Peoples R China
[2] Tongji Univ, Sch Life Sci & Technol, Dept Bioinformat, 1239 Siping Rd, Shanghai 200092, Peoples R China
关键词
invasion; NF‐ κ B pathway; Oral lichen planus; Prevotella melaninogenica; transcription; NECROSIS-FACTOR-ALPHA; PORPHYROMONAS-GINGIVALIS; MICROBIOTA; ACTIVATION; EXPRESSION; BINDING; TLR4; LPS;
D O I
10.1111/odi.13862
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective Oral lichen planus (OLP) is a chronic inflammatory disease that occurs in the oral mucosa with characteristic white striations lesions, recurrent erosions, and pains. The etiology and pathogenesis of OLP are still unclear. Materials and Methods We analyzed the bacterial community structure of buccal mucosa in patients with OLP and normal controls by high-throughput sequencing. Fluorescence in situ hybridization (FISH) was used to detect Prevotella melaninogenica (P. melaninogenica) in 13 OLP samples and 10 controls. The amounts of P. melaninogenica in OLP buccal mucosa and the expression of inflammatory cytokines in co-culture of mouse-derived macrophages with P. melaninogenica were detected by RT-qPCR. Results The P. melaninogenica was more abundant in OLP than in healthy controls, and the differences were significant at the level of the phylum, family, genus, and species (p < .05). FISH showed that P. melaninogenica can invade the epithelium and even the lamina propria of OLP, while no invasion was found in the normal mucosa. Prevotella melaninogenica can adhere to and invade macrophages and then activate the transcription of IL-1 beta, IL-6, and TNF-alpha in NF-kappa B signaling pathway. Conclusion Prevotella melaninogenica may be involved in the pathogenic process of OLP, and its specific mechanism deserves further study.
引用
收藏
页码:1580 / 1590
页数:11
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