Determining Seminal Plasma Human Immunodeficiency Virus Type 1 Load in the Context of Efficient Highly Active Antiretroviral Therapy

被引：23

作者：

Pasquier, Christophe ^{[1
,2
,3
]}

Saune, Karine ^{[2
,3
]}

Raymond, Stephanie ^{[2
,3
]}

Moinard, Nathalie ^{[4
,5
]}

Daudin, Myriam ^{[4
,5
]}

Bujan, Louis ^{[4
,5
]}

Izopet, Jacques ^{[2
,3
]}

机构：

[1] CHU Toulouse, Inst Federatif Biol, Serv Virol, TSA40031, F-31059 Toulouse, France

[2] Ctr Physiopathol Toulouse Purpan, INSERM, U563, F-31300 Toulouse, France

[3] Univ Toulouse 3, Fac Med & Pharm, F-31400 Toulouse, France

[4] Univ Toulouse 3, Human Fertil Res Grp, EA 3694, F-31059 Toulouse, France

[5] Hop Paule de Viguier, CHU Toulouse, CECOS Midi Pyrenees, F-31059 Toulouse, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 09期

关键词：

HIV-SERODISCORDANT COUPLES; MALE GENITAL-TRACT; VIRAL LOAD; HETEROSEXUAL TRANSMISSION; SEXUAL TRANSMISSION; DRUG CONCENTRATIONS; INFECTED PATIENTS; SEMEN; BLOOD; MEN;

D O I：

10.1128/JCM.02131-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to measure the HIV-1 load in semen plasma samples. HIV-1 was detected in 13.4% of the semen samples processed at our MAP center. Of the eight patients having a detectable semen HIV-1 load, five had no detectable virus in their blood plasma. This highlights the residual risk of HIV-1 transmission during unprotected intercourse and raises the question of the possible consequences of ineffective highly active antiretroviral therapy in the genital tract.

引用

收藏

页码：2883 / 2887

页数：5

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