A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques

被引：41

作者：

Li, Yuzhong ^{[1
]}

Bi, Yanwei ^{[1
]}

Xiao, Hongjian ^{[1
]}

Yao, Yueting ^{[1
]}

Liu, Xiaojuan ^{[1
]}

Hu, Zhengrong ^{[1
]}

Duan, Jinmei ^{[1
]}

Yang, Yaoyun ^{[1
]}

Li, Zhihua ^{[1
]}

Li, Yadong ^{[1
]}

Zhang, Heng ^{[1
]}

Ding, Chen ^{[1
]}

Yang, Jianbo ^{[1
]}

Li, Haiwei ^{[1
]}

He, Zhanlong ^{[1
]}

Liu, Longding ^{[1
]}

Hu, Guangnan ^{[2
]}

Liu, Shuying ^{[3
]}

Che, Yanchun ^{[1
]}

Wang, Shixia ^{[2
]}

Li, Qihan ^{[1
]}

Lu, Shan ^{[2
]}

Cun, Wei ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, 935 Jiaoling Rd, Kunming 650118, Yunnan, Peoples R China

[2] Univ Massachusetts, Med Sch, Dept Med, 364 Plantation St, Worcester, MA 01605 USA

[3] SYL Consulting, Thousand Oaks, CA USA

来源：

EMERGING MICROBES & INFECTIONS | 2021年 / 10卷 / 01期

关键词：

SARS-CoV-2; spike glycoprotein; DNA vaccine; protein vaccine; non-human primate; ANTIBODY-RESPONSES; INACTIVATED VACCINE; INFLUENZA; IMMUNOGENICITY; IMMUNIZATION; IDENTIFICATION; EXPRESSION; BOOST; MICE; H1;

D O I：

10.1080/22221751.2021.1887767

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.

引用

页码：342 / 355

页数：14

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