Next generation sequencing in cytology

被引：44

作者：

Pisapia, Pasquale ^{[1
]}

Pepe, Francesco ^{[1
]}

Sgariglia, Roberta ^{[1
]}

Nacchio, Mariantonia ^{[1
]}

Russo, Gianluca ^{[1
]}

Conticelli, Floriana ^{[1
]}

Girolami, Ilaria ^{[2
]}

Eccher, Albino ^{[3
,4
]}

Bellevicine, Claudio ^{[1
]}

Vigliar, Elena ^{[1
]}

Malapelle, Umberto ^{[1
]}

Troncone, Giancarlo ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Publ Hlth, Via Sergio Pansini 5, I-80131 Naples, Italy

[2] Cent Hosp Bolzano, Div Pathol, Bolzano, Italy

[3] Univ Verona, Dept Pathol & Diagnost, Verona, Italy

[4] Hosp Trust Verona, Verona, Italy

来源：

CYTOPATHOLOGY | 2021年 / 32卷 / 05期

关键词：

biomarkers; cytopathology; FNA; molecular cytopathology; next generation sequencing; FINE-NEEDLE-ASPIRATION; MOLECULAR TESTING GUIDELINE; WORLDWIDE RING TRIAL; LUNG-CANCER PATIENTS; DNA YIELD; SPECIMENS; CYTOPATHOLOGY; DIAGNOSIS; SAMPLES; BIOPSY;

D O I：

10.1111/cyt.12974

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high-quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre-analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre-analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.

引用

页码：588 / 595

页数：8

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