Next generation sequencing in cytology

被引：44
|
作者：
Pisapia, Pasquale [1 ]
Pepe, Francesco [1 ]
Sgariglia, Roberta [1 ]
Nacchio, Mariantonia [1 ]
Russo, Gianluca [1 ]
Conticelli, Floriana [1 ]
Girolami, Ilaria [2 ]
Eccher, Albino [3 ,4 ]
Bellevicine, Claudio [1 ]
Vigliar, Elena [1 ]
Malapelle, Umberto [1 ]
Troncone, Giancarlo [1 ]
机构：
[1] Univ Naples Federico II, Dept Publ Hlth, Via Sergio Pansini 5, I-80131 Naples, Italy
[2] Cent Hosp Bolzano, Div Pathol, Bolzano, Italy
[3] Univ Verona, Dept Pathol & Diagnost, Verona, Italy
[4] Hosp Trust Verona, Verona, Italy
来源：
CYTOPATHOLOGY | 2021年 / 32卷 / 05期
关键词：
biomarkers; cytopathology; FNA; molecular cytopathology; next generation sequencing; FINE-NEEDLE-ASPIRATION; MOLECULAR TESTING GUIDELINE; WORLDWIDE RING TRIAL; LUNG-CANCER PATIENTS; DNA YIELD; SPECIMENS; CYTOPATHOLOGY; DIAGNOSIS; SAMPLES; BIOPSY;
D O I：
10.1111/cyt.12974
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high-quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre-analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre-analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.
引用
收藏
页码：588 / 595
页数：8
相关论文
共 50 条
  • [1] Factors Affecting the Success of Next-Generation Sequencing in Cytology Specimens
    Roy-Chowdhuri, Sinchita
    Goswami, Rashmi S.
    Chen, Hui
    Patel, Keyur P.
    Routbort, Mark J.
    Singh, Rajesh R.
    Broaddus, Russell R.
    Barkoh, Bedia A.
    Manekia, Jawed
    Yao, Hui
    Medeiros, L. Jeffrey
    Staerkel, Gregg
    Luthra, Rajyalakshmi
    Stewart, John
    CANCER CYTOPATHOLOGY, 2015, 123 (11) : 659 - 668
  • [2] Use of cytology centrifuged supernatants improves cost and turnaround time for targeted next generation sequencing
    Gokozan, Hamza N.
    Harbhajanka, Aparna
    Bomeisl, Philip
    Michael, Claire W.
    Sadri, Navid
    DIAGNOSTIC CYTOPATHOLOGY, 2020, 48 (12) : 1167 - 1172
  • [3] Invited review-next-generation sequencing: a modern tool in cytopathology
    Roy-Chowdhuri, Sinchita
    Pisapia, Pasquale
    Salto-Tellez, Manuel
    Savic, Spasenija
    Nacchio, Mariantonia
    de Biases, Dario
    Tallini, Giovanni
    Troncone, Giancarlo
    Schmitt, Fernando
    VIRCHOWS ARCHIV, 2019, 475 (01) : 3 - 11
  • [4] Accuracy of next-generation sequencing for the identification of clinically relevant variants in cytology smears in lung adenocarcinoma
    Baum, Jordan E.
    Zhang, Pan
    Hoda, Rana S.
    Geraghty, Brian
    Rennert, Hanna
    Narula, Navneet
    Fernandes, Helen D.
    CANCER CYTOPATHOLOGY, 2017, 125 (06) : 398 - 406
  • [5] Comparison of cytocentrifugation supernatant fluid and formalin-fixed paraffin-embedded tissue for targeted next-generation sequencing
    Janaki, Nafiseh
    Harbhajanka, Aparna
    Michael, Claire W.
    Bomeisl, Phillip
    Wasman, Jay
    Atchley, Maureen
    Miskiewicz, Kristina
    Alouani, David
    Sadri, Navid
    CANCER CYTOPATHOLOGY, 2019, 127 (05) : 297 - 305
  • [6] Preanalytic Variables in Cytology Lessons Learned From Next-Generation Sequencing-The MD Anderson Experience
    Roy-Chowdhuri, Sinchita
    Stewart, John
    ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2016, 140 (11) : 1191 - 1199
  • [7] Next-generation sequencing improves the diagnosis of thyroid FNA specimens with indeterminate cytology
    Le Mercier, Marie
    D'Haene, Nicky
    De Neve, Nancy
    Blanchard, Oriane
    Degand, Caroline
    Rorive, Sandrine
    Salmon, Isabelle
    HISTOPATHOLOGY, 2015, 66 (02) : 215 - 224
  • [8] Comprehensive Validation of Cytology Specimens for Next-Generation Sequencing and Clinical Practice Experience
    Balla, Agnes
    Hampel, Ken J.
    Sharma, Mukesh K.
    Cottrell, Catherine E.
    Sidiropoulos, Nikoletta
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2018, 20 (06) : 812 - 821
  • [9] ?SMART? cytology: The next generation cytology for precision diagnosis
    Kim, Teresa
    Rao, Jianyu
    SEMINARS IN DIAGNOSTIC PATHOLOGY, 2023, 40 (02) : 95 - 99
  • [10] Next step of molecular pathology: next-generation sequencing in cytology
    da Silva, Ricella Souza
    Schmitt, Fernando
    JOURNAL OF PATHOLOGY AND TRANSLATIONAL MEDICINE, 2024, 58 (06) : 291 - 298
12345