Genetic diversity of Giardia isolates from patients in Chandigarh region: India

被引：1

作者：

Thakur, Shabnam ^{[1
]}

Kaur, Upninder ^{[1
]}

Sehgal, Rakesh ^{[1
]}

机构：

[1] Postgrad Inst Med Res & Educ, Dept Med Parasitol, Chandigarh 160012, India

来源：

BMC RESEARCH NOTES | 2021年 / 14卷 / 01期

关键词：

Giardia; Genotype; GLUD1 (glutamate dehydrogenase); PCR; Sequencing;

D O I：

10.1186/s13104-020-05419-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. Results Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.

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