Genetic diversity of Giardia isolates from patients in Chandigarh region: India

被引：1

作者：

Thakur, Shabnam ^{[1
]}

Kaur, Upninder ^{[1
]}

Sehgal, Rakesh ^{[1
]}

机构：

[1] Postgrad Inst Med Res & Educ, Dept Med Parasitol, Chandigarh 160012, India

来源：

BMC RESEARCH NOTES | 2021年 / 14卷 / 01期

关键词：

Giardia; Genotype; GLUD1 (glutamate dehydrogenase); PCR; Sequencing;

D O I：

10.1186/s13104-020-05419-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. Results Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.

引用

页数：5

共 50 条

[1] Genetic diversity of Giardia isolates from patients in Chandigarh region: India [J].

Shabnam Thakur ;

Upninder Kaur ;

Rakesh Sehgal .

BMC Research Notes, 14

[2] Genetic diversity of Cryptosporidium isolates from patients in North India [J].

Sharma, Poonam ;

Sharma, Aman ;

Sehgal, Rakesh ;

Malla, Nancy ;

Khurana, Sumeeta .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2013, 17 (08) :E601-E605

[3] Occurrence of Cryptosporidium and Giardia in potable water sources in Chandigarh, Northern India [J].

Utaaker, Kjersti Selstad ;

Joshi, Himanshu ;

Kumar, Anil ;

Chaudhary, Suman ;

Robertson, Lucy J. .

JOURNAL OF WATER SUPPLY RESEARCH AND TECHNOLOGY-AQUA, 2019, 68 (06) :483-494

[4] First genetic characterisation of Giardia in human isolates from Jordan [J].

Hijjawi, Nawal ;

Yang, Rongchang ;

Mukbel, Rami ;

Yassin, Yasmeen ;

Mharib, Taghrid ;

Ryan, Una .

PARASITOLOGY RESEARCH, 2016, 115 (10) :3723-3729

[5] Genetic identification of Giardia isolates from symptomatic and asymptomatic shelter dogs [J].

Papini, R. ;

Paoletti, B. ;

Cardini, G. ;

Giangaspero, A. .

REVUE DE MEDECINE VETERINAIRE, 2007, 158 (03) :143-147

[6] Genetic diversity and multiplicity of infection of Plasmodium falciparum isolates from Kolkata, West Bengal, India [J].

Saha, Pabitra ;

Ganguly, Swagata ;

Maji, Ardhendu K. .

INFECTION GENETICS AND EVOLUTION, 2016, 43 :239-244

[7] Genetic diversity of Orientia tsutsugamushi strains from patients in north India [J].

Kumar, Abhay ;

Biswal, Manisha ;

Zaman, Kamran ;

Sharma, Navneet ;

Suri, Vikas ;

Bhalla, Ashish .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2019, 84 :131-135

[8] Genotyping of Giardia intestinalis Isolates in the Thrace Region, Turkey [J].

Cicek, Cemal ;

Sakru, Nermin .

MIKROBIYOLOJI BULTENI, 2015, 49 (04) :576-585

[9] Molecular detection of potentially zoonotic protozoa in the Chandigarh region, India [J].

Rattan, Divya ;

Datta, Priya ;

Sharma, Devyani ;

Sharma, Shikha ;

Sehgal, Rakesh .

VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, 2024, 56

[10] Prevalence and genetic characterization of Giardia and Cryptosporidium in cats from Italy [J].

Paoletti, Barbara ;

Otranto, Domenico ;

Weigl, Stefania ;

Giangaspero, Annunziata ;

Di Cesare, Angela ;

Traversa, Donato .

RESEARCH IN VETERINARY SCIENCE, 2011, 91 (03) :397-399

← 1 2 3 4 5 →