Heterologous production of the Piromyces equi cinnamoyl esterase in Trichoderma reesei for biotechnological applications

被引：13
作者：
Poidevin, L. [1 ,2 ]
Levasseur, A. [1 ]
Paes, G. [1 ]
Navarro, D. [1 ]
Heiss-Blanquet, S. [2 ]
Asther, M. [1 ]
Record, E. [1 ]
机构：
[1] Univ Aix Marseille 2, Univ Aix Marseille 1, INRA, UMR1163, F-13288 Marseille 09, France
[2] Inst Francais Petr, Rueil Malmaison, France
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 49卷 / 06期
关键词：
biotechnology; cinnamoyl esterase; ferulic acid; heterologous production; Piromyces equi; Trichoderma reesei; FERULIC ACID ESTERASES; ASPERGILLUS-NIGER; DEGRADATION; PURIFICATION; CLASSIFICATION; RELEASE; COMPLEX; BRAN; TOOL;
D O I：
10.1111/j.1472-765X.2009.02734.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: The objective of the study was to produce and characterize the cinnamoyl esterase EstA from the anaerobic fungus Piromyces equi for potential industrial applications. Methods and Results: The catalytic domain EstA was produced in Trichoderma reesei. Because the two fungi displayed different genome features, including different codon usage and GC content, a synthetic gene was designed and expressed, leading to the production of the corresponding protein at around 33 mg per litre in the T. reesei culture medium. After the recombinant protein was purified, biochemical characterization showed that EstA presents peak activity at pH 6 center dot 5 and at 50-60 degrees C. Furthermore, EstA remained stable at pH 6-8 and below 50 degrees C. EstA was compared to cinnamoyl esterases FaeA and FaeB from Aspergillus niger in terms of ferulic acid (FA) release from wheat bran (WB), maize bran (MB) and sugar beet pulp (SBP). Conclusion: The synthetic gene was successfully cloned and overexpressed in T. reesei. EstA from P. equi was demonstrated to efficiently release FA from various natural substrates. Significance and Impact of the Study: Recombinant EstA produced in an industrial enzyme producer, T. reesei, was biochemically characterized, and its capacity to release an aromatic compound (FA) for biotechnological applications was demonstrated.
引用
收藏
页码：673 / 678
页数：6
相关论文
共 16 条
[1]   Technical advance in fungal biotechnology: development of a miniaturized culture method and an automated high-throughput screening [J].
Alberto, F. ;
Navarro, D. ;
de Vries, R. P. ;
Asther, M. ;
Record, E. .
LETTERS IN APPLIED MICROBIOLOGY, 2009, 49 (02) :278-282
[2]   Biotechnological applications and potential of fungal feruloyl esterases based on prevalence, classification and biochemical diversity [J].
Benoit, Isabelle ;
Danchin, Etienne G. J. ;
Bleichrodt, Robert-Jan ;
de Vries, Ronald P. .
BIOTECHNOLOGY LETTERS, 2008, 30 (03) :387-396
[3]   Feruloyl esterases as a tool for the release of phenolic compounds from agro-industrial by-products [J].
Benoit, Isabelle ;
Navarro, David ;
Marnet, Nathalie ;
Rakotomanomana, Nnjara ;
Lesage-Meessen, Laurence ;
Sigoillot, Jean-Claude ;
Asther, Marcel ;
Asther, Michele .
CARBOHYDRATE RESEARCH, 2006, 341 (11) :1820-1827
[4]   Functional classification of the microbial feruloyl esterases [J].
Crepin, VF ;
Faulds, CB ;
Connerton, IF .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 63 (06) :647-652
[5]   The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds [J].
de Vries, RP ;
VanKuyk, PA ;
Kester, HCM ;
Visser, J .
BIOCHEMICAL JOURNAL, 2002, 363 :377-386
[6]   The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides [J].
deVries, RP ;
Michelsen, B ;
Poulsen, CH ;
Kroon, PA ;
vandenHeuvel, RHH ;
Faulds, CB ;
Williamson, G ;
vandenHombergh, JPTW ;
Visser, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (12) :4638-4644
[7]   A modular cinnamoyl ester hydrolase from the anaerobic fungus Piromyces equi acts synergistically with xylanase and is part of a multiprotein cellulose-binding cellulase-hemicellulase complex [J].
Fillingham, IJ ;
Kroon, PA ;
Williamson, G ;
Gilbert, HJ ;
Hazlewood, GP .
BIOCHEMICAL JOURNAL, 1999, 343 :215-224
[8]   Purification and characterization of a feruloylesterase from Aspergillus awamori [J].
Koseki, T ;
Furuse, S ;
Iwano, K ;
Matsuzawa, H .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (10) :2032-2034
[9]   A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module [J].
Kroon, PA ;
Williamson, G ;
Fish, NM ;
Archer, DB ;
Belshaw, NJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (23) :6740-6752
[10]   Production of a chimeric enzyme tool associating the Trichoderma reesei swollenin with the Aspergillus niger feruloyl esterase A for release of ferulic acid [J].
Levasseur, Anthony ;
Saloheimo, Markku ;
Navarro, David ;
Andberg, Martina ;
Monot, Frederic ;
Nakari-Setala, Tiina ;
Asther, Marcel ;
Record, Eric .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 73 (04) :872-880
12