miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells

被引：30

作者：

Aida, Rieko ^{[1
]}

Hagiwara, Keitaro ^{[1
]}

Okano, Kazunori ^{[2
]}

Nakata, Kyoko ^{[1
]}

Obata, Yuho ^{[1
]}

Yamashita, Takahiro ^{[1
]}

Yoshida, Kaoru ^{[1
]}

Hagiwara, Hiromi ^{[1
]}

机构：

[1] Toin Univ Yokohama, Fac Biomed Engn, Aoba Ku, 1614 Kurogane Cho, Yokohama, Kanagawa 2258503, Japan

[2] Nara Inst Sci & Technol, Div Mat Sci, 8916-5 Takayama, Ikoma 6300192, Japan

来源：

MOLECULAR BIOLOGY REPORTS | 2021年 / 48卷 / 03期

关键词：

Apigenin; Human lung cancer cell A549; miR-34a-5p; SNAI1 (SNAIL); Apoptosis;

D O I：

10.1007/s11033-021-06255-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, and invasion and induces apoptosis in cultured lung cancer cells. However, there is little information on the involvement of microRNAs (miRNAs) in its effects. miRNA microarray analysis and polymerase-chain-reaction analysis of miRNAs revealed that treatment of human lung cancer A549 cells with apigenin up-regulated the level of miR-34a-5p. Furthermore, mRNA microarray analysis and the results of three microRNA target prediction tools showed that Snail Family Transcriptional Repressor 1 (SNAI1), which inhibits the induction of apoptosis, had its mRNA expression down-regulated in A549 cells treated with apigenin. Our findings suggest that apigenin might induce apoptosis by down-regulation of SNAI1 through up-regulation of miR-34a-5p in A549 cells.

引用

页码：2291 / 2297

页数：7

共 36 条

[1] Proteasomal degradation of the Fox01 transcriptional regulator in cells transformed by the P3k and Akt oncoproteins [J].