Functional topography of the fully grown human oocyte

被引：18
作者：
Monti, Manuela [1 ]
Calligaro, Alberto [2 ]
Behr, Barry [3 ]
Pera, Renee Rejo [4 ,5 ]
Redi, Carlo Alberto [6 ]
Wossidlo, Mark [4 ]
机构：
[1] San Matteo Fdn Hlth Hospitalizat & Care, Res Ctr Regenerat Med, Biotechnol Res Labs, Pavia, Italy
[2] Univ Pavia, Dept Publ Hlth Expt & Forens Med, Histol & Embryol Unit, Pavia, Italy
[3] Stanford Univ, Dept Obstet & Gynecol, Stanford, CA 94305 USA
[4] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA
[5] Montana State Univ, Dept Cell Biol & Neurosci, Bozeman, MT 59717 USA
[6] Univ Pavia, Dept Biol & Biotechnol L Spallanzani, Pavia, Italy
来源：
EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2017年 / 61卷 / 01期
关键词：
Human oocytes; human antral compartment; lipid droplets; SN oocytes; NSN oocytes; cytoplasmic lattices; transmission electron microscopy; CHROMATIN CONFIGURATION; MOUSE OOCYTES; MATURATION; ORGANIZATION; NUCLEAR;
D O I：
10.4081/ejh.2017.2769
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
In vivo maturation (IVM) of human oocytes is a technique used to increase the number of usable oocytes for in vitro fertilization (IVF) and represents a necessity for women with different ovarian pathologies. During IVM the oocytes progress from the germinal vesicle stage (GV) through the metaphase II and during this journey both nuclear and cytoplasmic rearrangements must be obtained to increase the probability to get viable and healthy zygotes/embryos after IVF. As the successful clinical outcomes of this technique are a reality, we wanted to investigate the causes behind oocytes maturation arrest. For obvious ethical reasons, we were able to analyze only few human immature oocytes discarded and donated to research by transmission electron microscopy showing that, as in the mouse, they have different chromatin and cytoplasmic organizations both essential for further embryo development.
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收藏
页码：32 / 35
页数：4
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