Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis

被引:21
作者
Jia, Honglin
Zhou, Jinlin
Ikadai, Hiromi
Matsuu, Aya
Suzuki, Hiroshi
Igarashi, Ikuo
Fujisaki, Kozo
Xuan, Xuenan
机构
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Kitasato Univ, Sch Vet Med & Anim Sci, Towada, Aomori 034, Japan
关键词
D O I
10.4269/ajtmh.2006.75.843
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens.
引用
收藏
页码:843 / 850
页数:8
相关论文
共 31 条
[1]   IMMUNOSUPPRESSION IN DOGS NATURALLY INFECTED WITH BABESIA-GIBSONI [J].
ADACHI, K ;
UENO, C ;
MAKIMURA, S .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 1993, 55 (03) :503-505
[2]  
ALIFRANGIS M, 2004, MALARIA J, V12, P3
[3]   Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs [J].
Benito, A ;
Carmena, D .
ACTA TROPICA, 2005, 95 (01) :9-15
[4]   An immunisation trial with in vitro produced Babesia bigemina exoantigens [J].
Beniwal, RP ;
Nichani, AK ;
Rakha, NK ;
Sharma, RD ;
Sarup, S .
TROPICAL ANIMAL HEALTH AND PRODUCTION, 1997, 29 (04) :124S-126S
[5]   Canine babesiosis [J].
Boozer, AL ;
Macintire, DK .
VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE, 2003, 33 (04) :885-+
[6]   Identification of Babesia bovis L-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis [J].
Bork, S ;
Okamura, M ;
Boonchit, S ;
Hirata, H ;
Yokoyama, N ;
Igarashi, I .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2004, 136 (02) :165-172
[7]   Canine piroplasmosis due to Babesia gibsoni:: clinical and morphological aspects [J].
Casapulla, R ;
Baldi, L ;
Avallone, V ;
Sannino, R ;
Pazzanese, L ;
Mizzoni, V .
VETERINARY RECORD, 1998, 142 (07) :168-169
[8]   Quantitative determination of sporozoites and circumsporozoite antigen in mosquitoes infected with Plasmodium falciparum or P-vivax [J].
De Arruda, ME ;
Collins, KM ;
Hochberg, LP ;
Ryan, PR ;
Wirtz, RA ;
Ryan, JR .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2004, 98 (02) :121-127
[9]   Estimation of the total parasite biomass in acute falciparum malaria from plasma PfHRP2 [J].
Dondorp, AM ;
Desakorn, V ;
Pongtavornpinyo, W ;
Sahassananda, D ;
Silamut, K ;
Chotivanich, K ;
Newton, PN ;
Pitisuttithum, P ;
Smithyman, AM ;
White, NJ ;
Day, NPJ .
PLOS MEDICINE, 2005, 2 (08) :788-797
[10]  
Fukumoto S, 2000, J PARASITOL, V86, P956, DOI 10.1645/0022-3395(2000)086[0956:MCOBGG]2.0.CO