Lower incidence of variants in Caladium bicolor Ait. plants propagated by culture of explants from younger tissue

被引:19
作者
Ahmed, EU [1 ]
Hayashi, T [1 ]
Zhu, Y [1 ]
Hosokawa, M [1 ]
Yazawa, S [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Agron & Hort Sci, Lab Vegetable & Ornamental Hort,Sakyo Ku, Kyoto 6068502, Japan
关键词
Caladium; explant; variant; micro-propagation;
D O I
10.1016/S0304-4238(02)00092-4
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Explants from various parts of Caladium bicolor Ait. plants were cultured on MS medium containing 1 ppm naphthalene acetic acid (NAA) and 1 ppm 6-benzyladenine (BA). Variations in the shape and color pattern were observed in the regenerated plants. In plants regenerated from shoot tip explants including the two youngest leaves, young leaves (third and fourth leaves from the top), unexpanded leaves (fifth or sixth leaves), expanded leaves and petiole, the frequency of the occurrence of variants was 14, 24, 41, 53 and 70%, respectively. Plants regenerated from young leaves of lateral buds or root tips also produced only a few variants (12-14%). Thus, explants from younger tissue having meristematic tissue produced fewer variations than explants from older tissue. The mode of variation (color pattern and shape of leaves) also varied with the age of the explant. We conclude that young tissue should be used to propagate Caladium plants by tissue culture. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:187 / 194
页数:8
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