The heterogenous nuclear riboprotein S1-1 regulates AT1 receptor gene expression via transcriptional and posttranscriptional mechanisms

被引：21

作者：

Mueller, Cornelius F. H. ^{[1
]}

Berger, Anja ^{[2
]}

Zimmer, Sebastian ^{[1
]}

Tiyerili, Vedat ^{[1
]}

Nickenig, Georg ^{[1
]}

机构：

[1] Univ Klinikum Bonn, Med Klin & Poliklin 2, D-53105 Bonn, Germany

[2] Univ Klinikum Saarlandes, Klin & Poliklin Innere Med 3, D-66421 Homburg, Germany

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 488卷 / 01期

关键词：

S1-1; protein; VSMC; Angiotensin II; Apoptosis; Proliferation; Regulation; mRNA; Transfection; Transcriptional regulation; mRNA stability; MESSENGER-RNA; ANGIOTENSIN-II; HNRNP D; PROTEINS; DESTABILIZATION; IDENTIFICATION;

D O I：

10.1016/j.abb.2009.06.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The AT1 receptor plays an essential role in the pathogenesis of atherosclerosis. AT1 receptor expression is predominately mediated via mRNA destabilization by mRNA binding proteins. We identified via MALDI-analysis the heterogenous nuclear riboprotein S1-1 as an important regulator of AT1 receptor mRNA stability. The S1-1 protein possesses multiple nucleolar and cellular functions in vascular smooth muscle cells (VSMC). Overexpression of S1-1 sense resulted in VSMC in significant stabilization of AT1 receptor mRNA. However, this stabilization of the AT1 receptor mRNA is accompanied by a significantly reduced AT1 receptor mRNA transcription as shown via nuclear run-on assay resulting finally in reduced AT1 receptor mRNA levels. Additionally, S1-1 overexpression leads to increased apoptosis in VSMC and decreases VSMC proliferation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：76 / 82

页数：7

共 20 条

[1] Identification of AUF-1 ligands reveals vast diversity of early response gene mRNAs [J].

Bhattacharya, S ;

Giordano, T ;

Brewer, G ;

Malter, JS .

NUCLEIC ACIDS RESEARCH, 1999, 27 (06) :1464-1472

[2] AU-RICH ELEMENTS - CHARACTERIZATION AND IMPORTANCE IN MESSENGER-RNA DEGRADATION [J].

CHEN, CYA ;

SHYU, AB .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (11) :465-470

[3] PRESENCE OF A UNIQUE SET OF NUCLEAR PROTEINS AT THE SITES SENSITIVE TO RNASE A AS WELL AS DNASE-I [J].

INOUE, A ;

HIGASHI, Y ;

HASUMA, T ;

YUKIOKA, M ;

MORISAWA, S .

JOURNAL OF BIOCHEMISTRY, 1986, 100 (03) :747-751

[4] Molecular cloning of a RNA binding protein, S1-1 [J].

Inoue, A ;

Takahashi, KP ;

Kimura, M ;

Watanabe, T ;

Morisawa, S .

NUCLEIC ACIDS RESEARCH, 1996, 24 (15) :2990-2997

[5]

INOUE A, 2004, BIOL CELL 0304

[6] hnRNP complexes: composition, structure, and function [J].

Krecic, AM ;

Swanson, MS .

CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (03) :363-371

[7]

LASSEGUE B, 1995, MOL PHARMACOL, V48, P601

[8] Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich element [J].

Loflin, P ;

Chen, CYA ;

Shyu, AB .

GENES & DEVELOPMENT, 1999, 13 (14) :1884-1897

[9] Atherosclerosis [J].

Lusis, AJ .

NATURE, 2000, 407 (6801) :233-241

[10] Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth [J].

Mueller, C ;

Baudler, S ;

Welzel, H ;

Böhm, M ;

Nickenig, G .

CIRCULATION, 2002, 105 (20) :2423-2428

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