Application of sage herbal dust essential oils and supercritical fluid extract for the growth control of Escherichia coli in minced pork during storage

Sage has various biological activity and long tradition of the using in phytopharmaceutical preparations and, recently, in food industry. In this work, the essential oil obtained by hydrodistillation and essential oil and extract obtained by supercritical fluid extraction from sage herbal dust, waste product of filter tea factory, were screened for antimicrobial activity against Escherichia coli in the medium which simulates the conditions in meat and in real system of minced pork. The supercritical fluid extract had much lower effect to the reduction of a number of E. coli in meat simulation medium. The total inhibition of E. coli growth was achieved after 12 h and 24 h with the addition of 0.6 mu l/ml of essential oil obtained by hydrodistilation and supercritical fluid extraction, respectively. The addition of essential oils and supercritical fluid extract of sage herbal dust could be used for the effective control of E. coli growth in minced meat.