Kinetic Characterization by Surface Plasmon Resonance-Based Biosensors: Principle and Emerging Trends

被引：26

作者：

De Crescenzo, Gregory ^{[1
]}

Boucher, Cyril ^{[1
,2
]}

Durocher, Yves ^{[2
]}

Jolicoeur, Mario ^{[1
]}

机构：

[1] Ecole Polytech, Dept Chem Engn, Inst Biomed Engn, Grp Rech Sci & Technol Biomed,Stn Ctr Ville, Montreal, PQ H3C 3A7, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Anim Cell Technol Grp, Bioproc Sector, Montreal, PQ H4P 2R2, Canada

来源：

CELLULAR AND MOLECULAR BIOENGINEERING | 2008年 / 1卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

SPR; Global analysis; Kinetic titration series; Coiled-coil; Affinity capture;

D O I：

10.1007/s12195-008-0035-5

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Within the last three decades, surface plasmon resonance (SPR)-based optical biosensors have emerged as leading instruments for the study of biomolecular interactions. The rapid dissemination of the technology has provided researchers with versatile and sensitive instruments for the identification of kinetic and thermodynamic parameters related to macromolecular interactions. This review article presents the basic principles, as well as the classical experimental approaches and data treatment techniques related to the use of commercially available instruments for the characterization of protein-protein interactions. Emphasis will be put on new experimental trends aiming at improving two important aspects of any SPR kinetic studies, i.e., proteins tethering on biosensor surfaces as well as the optimization of data collection.

引用

页码：204 / 215

页数：12

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