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Flow injection analysis with luminol chemiluminescence detection for evaluation of quenching effects of grape seed extracts and polyphenolics against reactive oxygen species
被引:5
作者:
Wada, Mitsuhiro
Katoh, Masayuki
Kido, Hirotsugu
Nakashima, Mihoko N.
Kuroda, Naotaka
Nakashima, Kenichiro
机构:
[1] Nagasaki Univ, Course Pharmaceut Sci, Grad Sch Biomed Sci, Nagasaki 8528521, Japan
[2] Mitsubishi Chem Corp, Specialty Chem Co, Aoba Ku, Yokohama, Kanagawa 2278502, Japan
关键词:
flow injection analysis;
chemiluminescent assay;
grape seed extract;
polyphenolics;
reactive oxygen species;
D O I:
10.2116/bunsekikagaku.55.931
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
A simple and rapid FIA method for measurement of quenching effects of grape seed extracts and polyphenolics against reactive oxygen species (ROS) was developed. This method is based on the determination of luminol chemiluminescence generated with ROS such as hydroxyl radical ((OH)-O-center dot), singlet oxygen (O-1(2)), peroxynitrite (ONOO-) and superoxide anion (O-2(-)). More than 1 sample/min could be measured with satisfactory precision. The total reagent amount in this method could be reduced to one tenth of that for our previous batch method. The grape seed extracts A and B were successfully evaluated by the proposed method. The EC50 of cyanidin as a representative polyphenolic against (OH)-O-center dot, O-1(2), ONOO center dot and O-2(-) were 50 +/- 4, 148 +/- 8, 36 +/- 4, and 95 +/- 8 ng/assay (n = 3), respectively. Except for chalcone, the other polyphenolics such as pelargonidin, delphinidin and trans-resveratrol also showed the high quenching effects against ROS.
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页码:931 / 936
页数:6
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