Biosynthesis of silver nanoparticles via fungal cell filtrate and their antiquorum sensing against Pseudomonas aeruginosa

In the present study, efficient, eco-friendly and sustainable green chemistry method was utilized for the synthesis of mycosilver nanoparticles by using mycelium filtrate of endophytic fungi Setosphaeria rostrata, isolated from the stem of Solanum nigrum. The mycosilver nanoparticle synthesis was confirmed by change in colour from light brown to dark brown. The synthesised mycosilver nano particles were characterized by UV-vis spectroscopy and the surface plasmon resonance was recorded at 400 nm. Analysis of AgNps by Fourier transform infrared spectroscopy (FTIR) indicated the presence of phenols, alkaloids, flavonoids and proteins which are responsible for reduction and capping for the mycosilver nanoparticles. Scanning Electron Microscopy (SEM),Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis(EDAX) images showed the agglomeration colloids with spherical shapes. The results of present study exhibited that mycosilver nanoparticles inhibited pyocyanin and extracellular polysaccharide (EPS) production, biofilm formation and swarming in Pseudomonas aeruginosa. The results suggested that mycosilver nanoparticles can be employed as a substitute to antibiotics to break antibiotic resistance in P. aeruginosa.