NMR and FTIR studies of hydrated pea proteins

被引:4
作者
Belton, PS [1 ]
Bogracheva, T [1 ]
Cserhalmi, Z [1 ]
Czukor, B [1 ]
Grant, A [1 ]
Lambert, N [1 ]
Wellner, N [1 ]
机构
[1] CENT FOOD RES INST, KEKI, H-1022 BUDAPEST, HUNGARY
关键词
D O I
10.1016/S0268-005X(97)80047-1
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The effects of increasing D2O hydration on the plasticization of vicilin, legumin and albumin fractions from peas were investigated using solid state H-1-NMR transverse relaxation techniques. Measurements. showed increases on hydration in the T-2 and intensity of the exponential component of the relaxation decay. However, a Gaussian (more rigid) component remained throughout the sample composition range. This behaviour contrasted with that observed in barley storage proteins and would indicate considerably less plasticization in legume proteins. In H-2-NMR transverse relaxation measurements of a highly D2O hydrated sample over a large temperature range, vicilin was shown to be hydrophilic in nature. However, the observed absorption of water by vicilin was less than in the HMW subunits of wheat. FTIR spectra show little structural change in vicilin and legumin on hydration, in contrast to changes occurring in the cereal proteins. These differences in behaviour may be ascribed to differences between the globular structure of the legume proteins and the more linear structure of the cereal proteins.
引用
收藏
页码:485 / 491
页数:7
相关论文
共 50 条
[41]   NMR and dielectric studies of hydrated collagen and elastin: Evidence for a delocalized secondary relaxation [J].
Lusceac, Sorin A. ;
Rosenstihl, Markus ;
Vogel, Michael ;
Gainaru, Catalin ;
Fillmer, Ariane ;
Boehmer, Roland .
JOURNAL OF NON-CRYSTALLINE SOLIDS, 2011, 357 (02) :655-663
[42]   CELL 191-Low resolution NMR studies of hydrated cellulosic fibres [J].
Ibbett, Roger N. ;
Schuster, K. Christian ;
Fasching, Mario ;
Zuckerstaetter, Gerhard .
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2008, 235
[43]   NMR studies of a new family of DNA binding proteins: the THAP proteins [J].
Virginie Gervais ;
Sébastien Campagne ;
Jade Durand ;
Isabelle Muller ;
Alain Milon .
Journal of Biomolecular NMR, 2013, 56 :3-15
[44]   NMR SPECTROSCOPIC STUDIES OF PARAMAGNETIC PROTEINS - IRON-SULFUR PROTEINS [J].
CHENG, H ;
MARKLEY, JL .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1995, 24 :209-237
[45]   NMR studies of a new family of DNA binding proteins: the THAP proteins [J].
Gervais, Virginie ;
Campagne, Sebastien ;
Durand, Jade ;
Muller, Isabelle ;
Milon, Alain .
JOURNAL OF BIOMOLECULAR NMR, 2013, 56 (01) :3-15
[46]   Starch gelatinization using sodium silicate: FTIR, DSC, XRPD, and NMR studies [J].
Rashid, Iyad ;
Al Omari, Mahmoud H. ;
Leharne, Stephen A. ;
Chowdhry, Babur Z. ;
Badwan, Adnan .
STARCH-STARKE, 2012, 64 (09) :713-728
[47]   C-13 CPMAS NMR AND FTIR SPECTROSCOPIC STUDIES OF HUMIC ACIDS [J].
GERASIMOWICZ, WV ;
BYLER, DM .
SOIL SCIENCE, 1985, 139 (03) :270-278
[48]   Solid state NMR and FTIR structural studies of cork cell walls: An overview [J].
Lopes, MH ;
Neto, CP ;
Gil, AM .
ADVANCES IN LIGNOCELLULOSICS CHEMISTRY FOR ECOLOGICALLY FRIENDLY PULPING AND BLEACHING TECHNOLOGIES, 1998, :357-360
[49]   Solid-state NMR and FTIR studies of CdS nanoparticle/polymer composites [J].
Ortiz-Colon, Saida Y. ;
Espe, Matthew ;
Ziolo, Ron F. ;
Manciu, Felicia S. .
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2007, 233 :389-389
[50]   FTIR AND NMR STUDIES OF ADSORBED CETHYLTRIMETHYLAMMONIUM CHLORIDE IN MCM-41 MATERIALS [J].
Taba, Paulina ;
Howe, Russell F. ;
Moran, Graine .
INDONESIAN JOURNAL OF CHEMISTRY, 2008, 8 (01) :1-6