Simultaneous Extraction of DNA and RNA from Hepatocellular Carcinoma (Hep G2) Based on Silica-Coated Magnetic Nanoparticles

被引：70

作者：

Wang, Jiuhai ^{[1
]}

Ali, Zeeshan ^{[1
]}

Si, Jin ^{[2
]}

Wang, Nianyue ^{[3
]}

He, Nongyue ^{[1
,4
]}

Li, Zhiyang ^{[5
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Peoples R China

[2] Nanjing Med Univ, Drum Tower Clin Coll, Dept Lab Med, Nanjing 210008, Peoples R China

[3] Second Hosp Nanjing, Clin Lab, Nanjing 210003, Peoples R China

[4] Hunan Univ Technol, Econ Forest Cultivat & Utilizat 2011 Collaborat I, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China

[5] Nanjing Med Univ, Affiliated Hosp 2, Dept Lab Med, Nanjing 210011, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2017年 / 17卷 / 01期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Nucleic Acids; Simultaneous Extraction; Magnetic Nanoparticles; Hep G2; NUCLEIC-ACIDS; GENOMIC DNA; PARTICLES; TISSUE; HYBRIDIZATION; MICROSPHERES; ADSORPTION; SEPARATION;

D O I：

10.1166/jnn.2017.12442

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nucleic acid (NA) extraction from cancer cells is an essential step in molecular oncologic testing. The conventional NA extraction protocols, based on several ultracentrifugation steps, suffer from time-consuming and complex manipulation. Here, a magnetic nanoparticle (MNP) based method for simultaneous extraction of DNA and RNA from cancer cells is described. This MNP based technique has received great attention and significant interest due to its convenient manipulation, low cost and ease for automation. Different factors including lysis buffer, ethanol, MNPs and washing buffers which may affect the yield of nucleic acid were optimized. The average yield of DNA and RNA obtained from 1 mL Hep G2 (similar to 10(6) cells) ranged from 9.7 to 14.7 mu g with A260/A280 values between 1.68 and 2.01. The isolated DNA and RNA, using this method, were suitable for downstream activities such as PCR and RT-PCR.

引用

页码：802 / 806

页数：5

共 44 条

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