Chemical Compositions, In-vitro Antioxidant, Anti-microbial, Anti-inflammatory and Cytotoxic Activities of Essential Oil of Acorus calamus L. Rhizome from North-East India

被引:60
作者
Loying, Rikraj
Gogoi, Roktim
Sarma, Neelav
Borah, Angana
Munda, Sunita
Pandey, Sudin Kumar
Lal, Mohan [1 ]
机构
[1] CSIR North East Inst Sci & Technol, Med Aromat & Econ Plants Grp, Jorhat 765006, Assam, India
关键词
Acorus calamus; beta-asarone; DPPH; essential oil; mitotic index; SWEET-FLAG; BETA-ASARONE; ANTIBACTERIAL ACTIVITY; LEAVES; IDENTIFICATION; ETHNOBOTANY; EXTRACTS; DISEASE; ROOTS; CELLS;
D O I
10.1080/0972060X.2019.1696236
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Acorus calamus L. is an important aromatic medicinal plant, which has many biological activities. The aim of this study was to evaluate the potential in-vitro antioxidant, anti-microbial, anti-inflammatory and cytotoxic activities of rhizome essential oil of A. calamus. Hydro-distillation process was used to extract the essential oil from dry rhizome using Clevenger apparatus. The chemical compounds identified through GC/MS analysis revealed that beta-asarone (82.42 %) as a major compound and calarene (2.41 %), euasarone (1.92 %), 2-methoxy-3-allylphenol (1.91 %) were minor compounds. The antioxidant activity of rhizome essential oil showed good free radical scavenging activity in DPPH assay (IC50=1.68 mu g/mL) as compared to standard, ascorbic acid (IC50=1.48 mu g/mL). Anti-microbial activity of rhizome essential oil showed the highest zone of inhibition against A. fumigates (16 mm) and lowest against S. cerevisiae (12 mm) at 500 mu g/mL concentration among the tested fungal strains whereas, for bacterial strains, the highest zone of inhibition was shown against B. subtilis (12 mm) and lowest against B. cereus (8 mm) at the same concentration. Anti-inflammatory assay showed the IC50 value was 172.41 mu g/mL whereas in the standard, sodium diclofenac, was 21.0 mu g/mL. The toxicity study in A. cepa roots showed that there was negligible effect on the mitotic index (MI=4.20 %) by the rhizome essential oil; as compared to the positive control, EMS (MI=14.79 %) at 1 mu L/mL. Based on these findings, it can be concluded that essential oil of A. calamus rhizome could serve as a natural antioxidant and anti-microbial source for commercial use.
引用
收藏
页码:1299 / 1312
页数:14
相关论文
共 68 条
[1]  
[Anonymous], 2012, Int. J. Curr. Sci
[2]  
[Anonymous], 1928, J AM PHARM ASS, DOI DOI 10.1002/JPS.3080170407
[3]   Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines [J].
Bains, JS ;
Dhuna, V ;
Singh, J ;
Kamboj, SS ;
Nijar, KK ;
Agrewala, JN .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2005, 5 (09) :1470-1478
[4]  
Bakare AA, 2000, J ENVIRON BIOL, V21, P263
[5]   Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East India [J].
Borah, Angana ;
Paw, Manabi ;
Gogoi, Roktim ;
Loying, Rikraj ;
Sarma, Neelav ;
Munda, Sunita ;
Pandey, Sudin Kumar ;
Lal, Mohan .
INDUSTRIAL CROPS AND PRODUCTS, 2019, 129 :448-454
[6]  
Cavazza G., 1976, Annales des Falsifications et de l'Expertise Chimique, V69, P833
[7]  
Chandra Sangita, 2012, Asian Pacific Journal of Tropical Biomedicine, V2, pS178
[8]  
Chowdhury A.R., 1997, INDIAN PERFUM, V41, P154
[9]  
CRAGG GM, 1994, CIBA F SYMP, V185, P178
[10]  
De M, 1999, PHYTOTHER RES, V13, P616, DOI 10.1002/(SICI)1099-1573(199911)13:7<616::AID-PTR475>3.0.CO