Single particle analysis: Methods for detection of platelet extracellular vesicles in suspension (excluding flow cytometry)

被引：28

作者：

Buzas, Edit I. ^{[1
]}

Gardiner, Chris ^{[2
]}

Lee, Changwon ^{[3
]}

Smith, Zachary J. ^{[4
]}

机构：

[1] Semmelweis Univ, Dept Genet Cell & Immunobiol, Nagyvarad Ter 4, H-1089 Budapest, Hungary

[2] UCL, Res Dept Hematol, Haemostasis Res Unit, London, England

[3] Univ Calif Davis, Med Ctr, Ctr Biophoton, Sacramento, CA 95817 USA

[4] Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, 443 Huangshan Rd, Hefei 230027, Anhui, Peoples R China

来源：

PLATELETS | 2017年 / 28卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Extracellular vesicles; exosomes; microvesicles; analytical methods; NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED MICROPARTICLES; RAMAN-SPECTROSCOPY; ACTIVATED PLATELETS; SIZE DISTRIBUTION; REFRACTIVE-INDEX; EXOSOMES; MICROVESICLES; SENSITIVITY; PLASMA;

D O I：

10.1080/09537104.2016.1260704

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) are small, membrane-bound particles released by all cell types, including abundant release by platelets. EVs are a topic of increasing interest in the academic and clinical community due to their increasingly recognised and diverse role in normal biology as well as in disease. However, typical analysis methods to characterise EVs released by cultured cells or isolated from whole blood or other body fluids are restricted to bulk analysis of all EVs in a sample. In this review, we discuss the motivation for analysis of individual EVs, as well as discuss three emerging methods for physical and chemical characterisation of individual EVs: nanoparticle tracking analysis, tunable resistive pulse sensing and Raman spectroscopy. We give brief descriptions of the working principles of each technique, along with a review noting the benefits and limitations of each method as applied to detection of single EVs.

引用

页码：249 / 255

页数：7

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