Sonochemical Decoration of Graphene Oxide with Magnetic Fe3O4@CuO Nanocomposite for Efficient Click Synthesis of Coumarin-Sugar Based Bioconjugates and Their Cytotoxic Activity

A magnetic nanocomposite of GO-Fe3O4@CuO was fabricated via a simple sonochemical technique and successfully utilized for the ultrasound promoted synthesis of regioselective 1,4-disubstituted mono/bis/tris-1,2,3-triazoles (3a-k). This catalyst could be separated conveniently from the reaction mixture using an external magnet and reused up to eight consecutive runs without noticeable drop in the desired product yield. Other noteworthy features of this green protocol are negligible metal leaching from the support during reaction, high yield in lesser time, aqueous media and good results with gram scale synthesis. Representative compounds were also screened for their cytotoxic activity against PC-12 cell line using standard MTT assay and flow cytometry. Trifluoromethyl group containing triazole derivative (3f) displayed cytotoxic activity (IC50 8.3 mu g/mL) comparable to the standard drug cisplatin (IC50 5.8 mu g/mL). [GRAPHICS] .