Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region

被引：3

作者：

Ai, Hong-Lian ^{[1
,2
]}

Wang, Hong ^{[1
,3
]}

Li, De-Zhu ^{[1
]}

Yang, Jun-Bo ^{[1
]}

机构：

[1] Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

[3] Nanjing Univ, Sch Life Sci, Nanjing 210093, Peoples R China

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 05期

关键词：

Incarvillea mairei; Genetic structure; Breeding system; Microsatellites; Himalaya-Hengduan Mountains region;

D O I：

10.1007/s10592-009-9808-1

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Incarvillea mairei (H. Leveille) Grierson (Bignoniaceae) is an endemic species to the Himalaya-Hengduan Mountains region. Here, we developed 13 microsatellite markers from I. mairei using a modified biotin-streptavidin capture method. Number of alleles per locus (NA) ranged from 3 to 7 with an average of 4.615. The observed (H-O) and expected (H-E) heterozygosities were from 0.050 to 0.800 and from 0.249 to 0.815, respectively. Additionally, among the 13 identified microsatellite markers, 12 of them were successfully amplified in other three congeneric species, and most of them showed polymorphic. Obtained evidences suggest that these markers provide a useful tool for further study of the population genetic structure and the breeding system in this species or/and infra-generic species.

引用

页码：1613 / 1615

页数：3

共 7 条

[1] Molecular phylogeny of Incarvillea (Bignoniaceae) based on its and trnL-F sequences [J].