Utilization of a Protein "Shuttle" To Load Vault Nanocapsules with Gold Probes and Proteins

被引：29

作者：

Goldsmith, Lisa E. ^{[1
]}

Pupols, Melody ^{[2
]}

Kickhoefer, Valerie A. ^{[2
]}

Rome, Leonard H. ^{[2
,3
]}

Monbouquette, Harold G. ^{[1
,3
]}

机构：

[1] Univ Calif Los Angeles, Chem & Biomol Engn Dept, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Biol Chem, David Geffen Sch Med, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

来源：

ACS NANO | 2009年 / 3卷 / 10期

基金：

美国国家科学基金会;

关键词：

vaults; protein shuttle; nanoencapsulation; drug delivery vehicle; gold clusters; DRUG-DELIVERY SYSTEMS; VIRUS-LIKE PARTICLES; TAGGED PROTEINS; RESISTANCE; NANOPARTICLE; RESOLUTION; THERAPY; DOMAIN; CELLS; VPARP;

D O I：

10.1021/nn900555d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Vaults are large protein nanocapsules that may be useful as drug delivery vehicles due to their normal presence in humans, their large interior volume, their simple structural composition consisting of multiple copies of one protein, and a recombinant production system that also provides a means to tailor their structure. However, for vaults to be effective in such applications, efficient means to load the interiors of the capsules must be demonstrated. Here we describe the use of a domain derived from a vault lumen-associated protein as a carrier to target both gold nanoclusters and heterologous His-tagged proteins to specific binding sites on the vault interior wall.

引用

页码：3175 / 3183

页数：9

共 38 条

[21] Artificial viruses: a nanotechnological approach to gene delivery [J].

Mastrobattista, E ;

van der Aa, MAEM ;

Hennink, WE ;

Crommelin, DJA .

NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (02) :115-121

[22] Cryoelectron microscopy imaging of recombinant and tissue derived vaults: Localization of the MVP N termini and VPARP [J].

Mikyas, Y ;

Makabi, M ;

Raval-Fernandes, S ;

Harrington, L ;

Kickhoefer, VA ;

Rome, LH ;

Stewart, PL .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 344 (01) :91-105

[23]

*NAN, 1998, NAN DET NAN LAB MOL

[24] Encapsulation of Semiconducting Polymers in Vault Protein Cages [J].

Ng, Benny C. ;

Yu, Marcella ;

Gopal, Ajaykumar ;

Rome, Leonard H. ;

Monbouquette, Harold G. ;

Tolbert, Sarah H. .

NANO LETTERS, 2008, 8 (10) :3503-3509

[25] Virus-like particles as immunogens [J].

Noad, R ;

Roy, P .

TRENDS IN MICROBIOLOGY, 2003, 11 (09) :438-444

[26] The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior [J].

Poderycki, Michael J. ;

Kickhoefer, Valerie A. ;

Kaddis, Catherine S. ;

Raval-Fernandes, Sujna ;

Johansson, Erik ;

Zink, Jeffrey I. ;

Loo, Joseph A. ;

Rome, Leonard H. .

BIOCHEMISTRY, 2006, 45 (39) :12184-12193

[27] Engineered metal binding sites on green fluorescence protein [J].

Richmond, TA ;

Takahashi, TT ;

Shimkhada, R ;

Bernsdorf, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 268 (02) :462-465

[28] THE DRUG RESISTANCE-RELATED PROTEIN LRP IS THE HUMAN MAJOR VAULT PROTEIN [J].

SCHEFFER, GL ;

WIJNGAARD, PLJ ;

FLENS, MJ ;

IZQUIERDO, MA ;

SLOVAK, ML ;

PINEDO, HM ;

MEIJER, CJLM ;

CLEVERS, HC ;

SCHEPER, RJ .

NATURE MEDICINE, 1995, 1 (06) :578-582

[29] AFFINITY PURIFICATION OF HISTIDINE-TAGGED PROTEINS [J].

SCHMITT, J ;

HESS, H ;

STUNNENBERG, HG .

MOLECULAR BIOLOGY REPORTS, 1993, 18 (03) :223-230

[30] Liposomes in drug delivery: progress and limitations [J].

Sharma, A ;

Sharma, US .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1997, 154 (02) :123-140

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