Coimmobilization of L-alpha-glycerophosphate oxidase with catalase and its application for the synthesis of dihydroxyacetone phosphate

The oxidation of L-alpha-glycerophosphate (1) to dihydroxyacetone phosphate (2) is carried out enzymatically under aerobic conditions by L-alpha-glycerophosphate oxidase coimmobilized with catalase, which serves for the decomposition of the generated hydrogen peroxide. 2 is trapped in an aldolase reaction with D/L-glyceraldehyde (3). The coimmobilized enzymes show an increased operational stability and can be reused in further transformations without loss of activity. (C) 1997 Elsevier Science Ltd.