Global DNA hypomethylation has no impact on lung function or serum inflammatory and fibrosis cytokines in asbestos-exposed population

被引:5
作者
Yu, Min [1 ,2 ]
Lou, Jianlin [2 ]
Xia, Hailing [2 ]
Zhang, Min [2 ]
Zhang, Yixiao [2 ]
Chen, Junqiang [2 ]
Zhang, Xing [2 ]
Ying, Shibo [2 ]
Zhu, Lijin [2 ]
Liu, Lihong [2 ]
Jia, Guang [1 ]
机构
[1] Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China
[2] Zhejiang Acad Med Sci, Dept Occupat Dis, 182 Tian Mu Shan Rd, Hangzhou 310013, Zhejiang, Peoples R China
关键词
Asbestos; Fibrosis; Global DNA methylation; Lung function; CCL5; CC16; PLEURAL PLAQUES; PULMONARY-FUNCTION; METHYLATION LEVELS; PROTEIN CC16; TNF-ALPHA; BLOOD DNA; ASSOCIATION; WORKERS; ADULTS; SPIROMETRY;
D O I
10.1007/s00420-017-1195-1
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
To examine the effect of asbestos exposure on global DNA methylation and determine whether lung function and inflammatory and fibrosis biomarkers are correlated with the methylation state. A total of 26 healthy subjects without asbestos exposure (Group 1), 47 healthy subjects with exposure (Group 2), and 52 subjects with benign asbestos-related disorders (ARDs) (Group 3) participated in this cross-sectional study. Blood global 5-methylcytosine (5mC) and serum TNF-alpha, collagen IV, CCL5 and CC16 concentrations were analyzed using enzyme-linked immunosorbent assay-like assays. Spirometric maneuvers were performed to assess lung function. Decreased 5mC levels were observed in Groups 2 and 3 compared to Group 1, irrespective of lung function (p < 0.01). There was no significant change in 5mC between Groups 2 and 3. Overall, 5mC was negatively correlated with CCL5 and collagen IV (p < 0.05), but no significant inverse relationship was found between 5mC and CCL5 or collagen IV in each group. Additionally, both 5mC and CC16 were inversely associated with FEV1/FVC% (p = 0.001, adjusted R (2) = 0.145) for non-smokers, and consistently significant inverse relationships were found between CC16 and FEV1/FVC%, independent of asbestos exposure. Asbestos exposure causes global DNA hypomethylation. DNA hypomethylation has no influence on serum biomarkers and lung function in asbestos-exposed population with or without pleural and pulmonary parenchymal abnormalities.
引用
收藏
页码:265 / 274
页数:10
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