Soluble, folded and active subtilisin in a protic ionic liquid

被引：18

作者：

Falcioni, Francesco ^{[1
]}

Housden, Hazel R. ^{[1
]}

Ling, Zhenlian ^{[1
]}

Shimizu, Seishi ^{[2
]}

Walker, Adam J. ^{[1
]}

Bruce, Neil C. ^{[1
]}

机构：

[1] Univ York, Dept Biol, CNAP, York YO10 5YW, N Yorkshire, England

[2] Univ York, Dept Chem, York Struct Biol Lab, York YO10 5YW, N Yorkshire, England

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

CATALYSIS;

D O I：

10.1039/b916497a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.

引用

页码：749 / 751

页数：3

共 14 条

[11] Ionic liquid salt-induced inactivation and unfolding of cellulase from Trichoderma reesei [J].

Turner, MB ;

Spear, SK ;

Huddleston, JG ;

Holbrey, JD ;

Rogers, RD .

GREEN CHEMISTRY, 2003, 5 (04) :443-447

[12] Cofactor-dependent enzyme catalysis in functionalized ionic solvents [J].

Walker, AJ ;

Bruce, NC .

CHEMICAL COMMUNICATIONS, 2004, (22) :2570-2571

[13] Room-temperature ionic liquids. Solvents for synthesis and catalysis [J].

Welton, T .

CHEMICAL REVIEWS, 1999, 99 (08) :2071-2083

[14] Negative effect of [bmim] [PF6] on the catalytic activity of alcohol dehydrogenase:: mechanism and prevention [J].

Zhang, Yun ;

Huang, Xirong ;

Li, Yuezhong .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (09) :1230-1235

← 1 2 →