Comparison of quantum dots immunofluorescence histochemistry and conventional immunohistochemistry for the detection of caveolin-1 and PCNA in the lung cancer tissue microarray

被引：64

作者：

Chen, Honglei ^{[1
,2
]}

Xue, Jingling ^{[2
]}

Zhang, Yuxia ^{[2
]}

Zhu, Xiaobo ^{[3
]}

Gao, Jun ^{[2
]}

Yu, Baoping ^{[1
]}

机构：

[1] Wuhan Univ, Renmin Hosp, Dept Internal Med, Wuhan 430060, Peoples R China

[2] Wuhan Univ, Sch Basic Med Sci, Dept Pathol, Wuhan 430071, Peoples R China

[3] Wuhan Tumor Nanometer Diag Engn Res Ctr, Wuhan 430072, Peoples R China

来源：

JOURNAL OF MOLECULAR HISTOLOGY | 2009年 / 40卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Lung cancer; Caveolin-1; PCNA; Quantum dots; Tissue microarray; LASER-SCANNING MICROSCOPY; EXPRESSION; TARGETS; PROBES; HER2;

D O I：

10.1007/s10735-009-9237-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Luminescent semiconductor quantum dots (QDs) are a new class of fluorescent label with wide ranges of applications in cell imaging. In this study, we evaluated the capability of QDs immunofluorescence histochemistry (QDs-IHC) for detecting antigens of caveolin-1 and PCNA in the lung cancer tissue microarray (TMA) in comparison with the conventional immunohistochemistry (IHC) technique. Both methods revealed consistent antigen localization and statistically non-significant detection rates of caveolin-1 and PCNA expressions in our study. However, the sensitivity of QDs-IHC was higher than IHC. The positive detection rates of caveolin-1 and PCNA by QDs-IHC were 57% (40/70) and 86% (60/70), respectively, which were higher than the detection rates of 47% (33/70) and 77% (54/70), respectively, by IHC. Moreover, QDs exhibited a much better photostability, a broader excitation spectrum and a longer fluorescence lifetime. We showed here the advantages of QDs-IHC over IHC for the detection of caveolin-1 and PCNA in lung cancer TMA.

引用

页码：261 / 268

页数：8

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