Toxoplasma gondii:: Comparison of human CD34+ and monocyte-derived dendritic cells after parasite infection

被引：1
作者：
Persat, F.
Diana, J.
Benadiba, C.
Ferrandiz, J.
Peguet-Navarro, J.
Peyron, F.
Picot, S.
Schmitt, D.
Vincent, C.
机构：
[1] Univ Lyon 1, Lab Parasitol Mycol Med & Pathol Exot, EA 3732, F-69373 Lyon 08, France
[2] Hop Edouard Herriot, Lab Cellules Langerhans Peau Humaine & Immun, F-69437 Lyon 03, France
来源：
EXPERIMENTAL PARASITOLOGY | 2007年 / 115卷 / 01期
关键词：
Toxoplasma gondii; human dendritic cells; CD34(+)-derived DC; monocyte-derived DC; DC maturation; DC migration; parasite infection; dendritic cells; monocyte-derived dendritic cells; CD34(+)-derived dendritic cells; granulocyte macrophage colony stimulating factor; lipopolysaccharide; type 1 surface antigen;
D O I：
10.1016/j.exppara.2006.06.003
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Human dendritic cells (DC) obtained in vitro from CD34(+) progenitors (CD34-DC) or blood monocytes (mo-DC) are different DC which may be used in a model of T gondii infection. We compared the survival, infection rate and cell surface receptor expression of both DC types after living T gondii tachyzoite infection. CD34-DC appeared less resistant to the parasite than mo-DC. At 48 h post-infection, chemokine receptors responsible for DC homing and migration were absent in mo-DC, while down regulation of CCR6 and up regulation of CCR7 was observed in CD34-DC. This result, suggesting migration ability of CD34-DC, was confirmed by in vitro migration experiments against different chemokines. Tachyzoite supernatant, used as chemokine, attracted immature CD34-DC as observed by MIP3 alpha, while MIP3 beta, as expected, attracted mature CD34-DC. Under similar conditions, no significant difference was noticed between mature or immature mo-DC. These data indicated that CD34-DC represent an alternative model that allows migration assay of infected DC by T gondii. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：103 / 106
页数：4
相关论文
共 27 条
[1]   Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells [J].
Alberti, J ;
Valenzuela, JG ;
Carruthers, VB ;
Hieny, S ;
Andersen, J ;
Charest, H ;
Sousa, CRE ;
Fairlamb, A ;
Ribeiro, JM ;
Sher, A .
NATURE IMMUNOLOGY, 2003, 4 (05) :485-490
[2]   Turning it on and off:: regulation of dendritic cell function in Toxoplasma gondii infection [J].
Aliberti, J ;
Jankovic, D ;
Sher, A .
IMMUNOLOGICAL REVIEWS, 2004, 201 :26-34
[3]   Origin and differentiation of dendritic cells [J].
Ardavín, C ;
del Hoyo, GM ;
Martín, P ;
Anjuère, F ;
Arias, CF ;
Marín, AR ;
Ruiz, S ;
Parrillas, V ;
Hernández, H .
TRENDS IN IMMUNOLOGY, 2001, 22 (12) :691-700
[4]   Subcellular localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid raft-associated form [J].
Assossou, O ;
Besson, F ;
Rouault, JP ;
Persat, F ;
Brisson, C ;
Duret, L ;
Ferrandiz, J ;
Mayençon, M ;
Peyron, F ;
Picot, S .
FEMS MICROBIOLOGY LETTERS, 2003, 224 (02) :161-168
[5]   Differential infectivity and division of Toxoplasma gondii in human peripheral blood leukocytes [J].
Channon, JY ;
Seguin, RM ;
Kasper, LH .
INFECTION AND IMMUNITY, 2000, 68 (08) :4822-4826
[6]   CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain [J].
Courret, N ;
Darche, S ;
Sonigo, P ;
Milon, G ;
Buzoni-Gâtel, D ;
Tardieux, I .
BLOOD, 2006, 107 (01) :309-316
[7]   Migration and maturation of human dendritic cells infected with Toxoplasma gondii depend on parasite strain type [J].
Diana, J ;
Persat, F ;
Staquet, MJ ;
Assossou, O ;
Ferrandiz, J ;
Gariazzo, MJ ;
Peyron, F ;
Picot, S ;
Schmitt, D ;
Vincent, C .
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2004, 42 (03) :321-331
[8]   Selective recruitment of immature and mature dendritic cells by distinct chemokines expressed in different anatomic sites [J].
Dieu, MC ;
Vanbervliet, B ;
Vicari, A ;
Bridon, JM ;
Oldham, E ;
Aït-Yahia, S ;
Brière, F ;
Zlotnik, A ;
Lebecque, S ;
Caux, C .
JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (02) :373-386
[9]   INFECTION OF MURINE PERITONEAL-MACROPHAGES WITH TOXOPLASMA-GONDII EXPOSED TO ULTRAVIOLET-LIGHT [J].
ENDO, T ;
PELSTER, B ;
PIEKARSKI, G .
ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 1981, 65 (02) :121-129
[10]   Flow cytometric quantification of Toxoplasma gondii cellular infection and replication [J].
Gay-Andrieu, F ;
Cozon, GJN ;
Ferrandiz, J ;
Kahi, S ;
Peyron, F .
JOURNAL OF PARASITOLOGY, 1999, 85 (03) :545-549
123