Characterization of Microbial Communities in a Dairy Farm Matrix in Ningxia, China, by 16S rDNA Analysis

被引:6
作者
Zhang, Wen [1 ,2 ]
Li, Wu [1 ,2 ]
Ma, Chenjie [1 ,2 ]
Wu, Xiaoling [1 ,2 ]
Li, Xunde [3 ,4 ]
Zeng, Jin [1 ,2 ]
Deng, Guangcun [1 ,2 ]
Wang, Yujiong [1 ,2 ]
机构
[1] Minist Educ Conservat & Utilizat Special Biol Res, Key Lab, Yinchuan, Peoples R China
[2] Ningxia Univ, Coll Life Sci, Yinchuan 750021, Ningxia, Peoples R China
[3] Western Inst Food Safety & Secur, 1477 Drew Ave,Suite 101, Davis, CA 95616 USA
[4] Dept Populat Hlth & Reprod, 1089 Vet Med Dr, Davis, CA 95616 USA
关键词
ESCHERICHIA-COLI O157H7; STAPHYLOCOCCUS-AUREUS; BOVINE MASTITIS; TETRACYCLINE RESISTANCE; BACTERIAL DIVERSITY; SALMONELLA-ENTERICA; RARE BIOSPHERE; SEQUENCE DATA; DIVISION TM7; ANIMAL FEED;
D O I
10.1155/2019/3827360
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A large amount of dairy manure is produced annually in the Ningxia Hui Autonomous Region of China due to the increase in food-producing animal agriculture in this region. The presence of bovine-originated zoonotic, especially human, pathogenic bacteria in untreated manure poses a significant threat to the environment and to public health. However, little is known about the composition, diversity, and abundance of bacterial communities in untreated dairy manure in the Ningxia region. In this study, the microbial community structure of the dairy farm matrix was characterized through 16S rDNA sequencing. The impact of manure treatment methods on bacterial communities was also analyzed. The results showed that the microbial community in dairy manure contained both beneficial bacteria and pathogens, with Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, and Actinobacteria as dominant phyla. The results also showed the diversity and variety of abundance of zoonotic pathogens among different matrices. The number of pathogens was found to increase significantly in the accumulated but untreated manure, which appeared to be the main matrix of dairy farms that accumulated pathogens including zoonotic pathogens. Findings from this study suggested that farm management, particularly proper treatment of manure, is essential to achieve a shift in the bacterial community composition and a reduction in the environmental load of pathogens including zoonotic pathogens.
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页数:13
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