Hydrogel derived from decellularized porcine adipose tissue as a promising biomaterial for soft tissue augmentation

被引：57
作者：
Tan, Qiu-Wen [1 ,2 ]
Zhang, Yi [1 ]
Luo, Jing-Cong [2 ]
Zhang, Di [1 ]
Xiong, Bin-Jun [1 ]
Yang, Ji-Qiao [1 ]
Xie, Hui-Qi [2 ]
Lv, Qing [1 ]
机构：
[1] Sichuan Univ, West China Hosp, Dept Breast Surg, Chengdu 610041, Sichuan, Peoples R China
[2] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Lab Stem Cell & Tissue Engn, Chengdu 610041, Sichuan, Peoples R China
来源：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2017年 / 105卷 / 06期
关键词：
adipogenesis; adipose-derived stem cell; ECM scaffold; hydrogel; tissue engineering; EXTRACELLULAR-MATRIX; MECHANICAL-PROPERTIES; COLLAGEN; XENOTRANSPLANTATION; ADIPOGENESIS; ANGIOGENESIS; SCAFFOLD; PROVIDE; CELLS;
D O I：
10.1002/jbm.a.36025
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Decellularized extracellular matrix (ECM) scaffolds from human adipose tissue, characterized by impressive adipogenic induction ability, are promising for soft tissue augmentation. However, scaffolds from autologous human adipose tissue are limited by the availability of tissue resources and the time necessary for scaffold fabrication. The objective of the current study was to investigate the adipogenic properties of hydrogels of decellularized porcine adipose tissue (HDPA). HDPA induced the adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro, with significantly increased expression of adipogenic genes. Subcutaneous injection of HDPA in immunocompetent mice induced host-derived adipogenesis without cell seeding, and adipogenesis was significantly enhanced with ADSCs seeding. The newly formed adipocytes were frequently located on the basal side in the non-seeding group, but this trend was not observed in the ADSCs seeding group. Our results indicated that, similar to human adipose tissue, the ECM scaffold derived from porcine adipose tissue could provide an adipogenic microenvironment for adipose tissue regeneration and is a promising biomaterial for soft tissue augmentation. (C) 2017 Wiley Periodicals, Inc.
引用
收藏
页码：1756 / 1764
页数：9
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