Role of NKG2D in cytokine-induced killer cells against lung cancer

被引:6
作者
Yin, Xiaowei [1 ]
Lu, Xuzhang [2 ]
Zhang Xiuwen [2 ]
Min, Zhou [3 ]
Xiao, Rong [2 ]
Mao, Zhengdao [1 ]
Zhang, Qian [1 ]
机构
[1] Nanjing Med Univ, Dept Resp Med, Changzhou Peoples Hosp 2, 29 Xinglong Alley, Changzhou 213000, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Hematol, Changzhou Peoples Hosp 2, Changzhou 213000, Jiangsu, Peoples R China
[3] Changzhou 3 Peoples Hosp, Dept Hematol, Changzhou 213000, Jiangsu, Peoples R China
关键词
NKG2D ligand; cytokine-induced killer cells; lung cancer; MICA/B; I-RELATED CHAIN; MEDIATED CYTOLYSIS; LIGAND EXPRESSION; LEUKEMIC-CELLS; CYTOTOXICITY; RECEPTOR; SUSCEPTIBILITY; IMMUNOTHERAPY; RECOGNITION; MICA;
D O I
10.3892/ol.2017.5800
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
It has been previously demonstrated that cytokine-induced killer (CIK) cells possess potent cytotoxicity against various cancer cells, including lung cancer cells. However, the mechanism by which CIK cells recognize lung cancer cells has not been understood. The interaction between killer cell lectin like receptor K1 (NKG2D) receptor and NKG2D ligands was demonstrated to serve an important role in target cell killing by natural killer cells. The present study investigated whether NKG2D receptor and NKG2D ligand interactions are involved in the CIK-directed killing of lung cancer cells. The expression of MICA and ULBP2 was detected in tumor and healthy tissue samples. The expression of MICA and ULBP2 in tumor tissue samples was higher compared with that in the healthy control tissue. The expression of NKG2D ligands was analyzed in A549 and Q56 cells through reverse transcription-quantitative polymerase chain reaction and flow cytometry. The results demonstrated that the lung cancer cell lines markedly expressed the NKG2D ligands. Furthermore, NKG2D ligand-expressing lung cancer cells were targeted by CIK cells, which was partially blocked by treating CIK cells with an antibody against NKG2D. The data of the current study has demonstrated that the NKG2D-NKG2D ligand interaction serves an essential role in mediating lung cancer cell killing by CIK cells.
引用
收藏
页码:3139 / 3143
页数:5
相关论文
共 24 条
[1]   Importance of NKG2D-NKG2D ligands interaction for cytolytic activity of natural killer cell [J].
Bae, Deok Sung ;
Hwang, Yu Kyeong ;
Lee, Jae Kwon .
CELLULAR IMMUNOLOGY, 2012, 276 (1-2) :122-127
[2]   HLA class I, NKG2D, and natural cytotoxicity receptors regulate multiple myeloma cell recognition by natural killer cells [J].
Carbone, E ;
Neri, P ;
Mesuraca, M ;
Fulciniti, MT ;
Otsuki, T ;
Pende, D ;
Groh, V ;
Spies, T ;
Pollio, G ;
Cosman, D ;
Catalano, L ;
Tassone, P ;
Rotoli, B ;
Venuta, S .
BLOOD, 2005, 105 (01) :251-258
[3]  
Champsaur M, 2010, IMMUNOL REV, V235, P267, DOI 10.1111/j.0105-2896.2010.00893.x
[4]   The role of the NKG2D receptor for tumor immunity [J].
Coudert, Jerome D. ;
Held, Werner .
SEMINARS IN CANCER BIOLOGY, 2006, 16 (05) :333-343
[5]   Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012 [J].
Ferlay, Jacques ;
Soerjomataram, Isabelle ;
Dikshit, Rajesh ;
Eser, Sultan ;
Mathers, Colin ;
Rebelo, Marise ;
Parkin, Donald Maxwell ;
Forman, David ;
Bray, Freddie .
INTERNATIONAL JOURNAL OF CANCER, 2015, 136 (05) :E359-E386
[6]  
Friese MA, 2003, CANCER RES, V63, P8996
[7]   Intracellular retention of the NKG2D ligand MHC class I chain-related gene a in human melanomas confers immune privilege and prevents NK cell-mediated cytotoxicity [J].
Fuertes, Mercedes B. ;
Girart, Maria V. ;
Molinero, Luciana L. ;
Domaica, Carolina I. ;
Rossi, Lucas E. ;
Barrio, Maria M. ;
Mordoh, Jose ;
Rabinovich, Gabriel A. ;
Zwirner, Norberto W. .
JOURNAL OF IMMUNOLOGY, 2008, 180 (07) :4606-4614
[8]   Maintenance Chemotherapy for Advanced Non-Small-Cell Lung Cancer: New Life for an Old Idea [J].
Gerber, David E. ;
Schiller, Joan H. .
JOURNAL OF CLINICAL ONCOLOGY, 2013, 31 (08) :1009-1020
[9]   Randomized Phase II Trial of Single-Agent Amrubicin or Topotecan as Second-Line Treatment in Patients With Small-Cell Lung Cancer Sensitive to First-Line Platinum-Based Chemotherapy [J].
Jotte, Robert ;
Conkling, Paul ;
Reynolds, Craig ;
Galsky, Matthew D. ;
Klein, Leonard ;
Fitzgibbons, James F. ;
McNally, Richard ;
Renschler, Markus F. ;
Oliver, Jennifer W. .
JOURNAL OF CLINICAL ONCOLOGY, 2011, 29 (03) :287-293
[10]   Regulation of the expression of MHC class I-related chain A, B (MICA, MICB) via chromatin remodeling and its impact on the susceptibility of leukemic cells to the cytotoxicity of NKG2D-expressing cells [J].
Kato, N. ;
Tanaka, J. ;
Sugita, J. ;
Toubai, T. ;
Miura, Y. ;
Ibata, M. ;
Syono, Y. ;
Ota, S. ;
Kondo, T. ;
Asaka, M. ;
Imamura, M. .
LEUKEMIA, 2007, 21 (10) :2103-2108