Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification

被引:100
作者
Kostyuk, Alexander I. [1 ,2 ]
Demidovich, Aleksandra D. [1 ]
Kotova, Daria A. [1 ]
Belousov, Vsevolod V. [1 ,2 ,3 ]
Bilan, Dmitry S. [1 ,2 ]
机构
[1] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Pirogov Russian Natl Res Med Univ, Moscow 117997, Russia
[3] Georg August Univ Gottingen, Inst Cardiovasc Physiol, D-37073 Gottingen, Germany
基金
俄罗斯科学基金会; 俄罗斯基础研究基金会;
关键词
genetically encoded biosensors; circularly permuted fluorescent proteins; GCAMP CALCIUM INDICATOR; NADH-NAD(+) REDOX STATE; LIVING CELLS; KINASE-C; STRUCTURAL BASIS; CA2+ INDICATORS; REACTIVE OXYGEN; DYNAMIC-RANGE; CRYSTAL-STRUCTURE; NEURAL ACTIVITY;
D O I
10.3390/ijms20174200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class of biosensors that allow the monitoring of many intracellular events. In circularly permuted FPs (cpFPs), the original N- and C-termini are fused using a peptide linker, while new termini are formed near the chromophore. Such a structure imparts greater mobility to the FP than that of the native variant, allowing greater lability of the spectral characteristics. One of the common principles of creating genetically encoded biosensors is based on the integration of a cpFP into a flexible region of a sensory domain or between two interacting domains, which are selected according to certain characteristics. Conformational rearrangements of the sensory domain associated with ligand interaction or changes in the cellular parameter are transferred to the cpFP, changing the chromophore environment. In this review, we highlight the basic principles of such sensors, the history of their creation, and a complete classification of the available biosensors.
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页数:37
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