Comparison of Trypsin Inhibitors in Preservation Solution for Islet Isolation

被引：15

作者：

Noguchi, Hirofumi ^{[1
,2
,3
,4
]}

Ueda, Michiko ^{[4
]}

Hayashi, Shuji ^{[3
]}

Kobayashi, Naoya ^{[5
]}

Okitsu, Teru ^{[2
]}

Iwanaga, Yasuhiro ^{[2
]}

Nagata, Hideo ^{[4
]}

Liu, Xiaoling ^{[4
]}

Kamiya, Hiroki ^{[4
]}

Levy, Marlon F. ^{[1
]}

Matsumoto, Shinichi ^{[1
,4
]}

机构：

[1] Baylor Res Inst, Baylor Inst Immunol Res, Baylor All St Med Ctr, Dallas, TX 75204 USA

[2] Kyoto Univ Hosp, Transplantat Unit, Kyoto 6068507, Japan

[3] Nagoya Univ, Grad Sch Med, Dept Adv Med Biotechnol & Robot, Nagoya, Aichi 4668550, Japan

[4] Fujita Hlth Univ, Dept Surg 2, Aichi 4701192, Japan

[5] Okayama Univ, Grad Sch Med & Dent, Dept Surg, Okayama 7008558, Japan

来源：

CELL TRANSPLANTATION | 2009年 / 18卷 / 5-6期

关键词：

Islet transplantation; Islet isolation; MK solution; Trypsin inhibitor; Preservation solution; CLINICAL LUNG TRANSPLANTATION; OF-WISCONSIN SOLUTION; HEART-BEATING DONORS; ET-KYOTO SOLUTION; PANCREAS PRESERVATION; BETA2/NEUROD PROTEIN; YIELDS; ULINASTATIN; NEUTROPHIL; ACTIVATION;

D O I：

10.1177/096368970901805-609

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Islet transplantation has recently emerged as an effective therapy and potential cure for type I diabetes mellitus. Recent reports show that the two-layer method (TLM), which employs oxygenated perfluorochemical (PFC) and University of Wisconsin (UW) solution, is superior to simple cold storage in UW for pancreas preservation in islet transplantation. Moreover, we recently reported that islet yield was significantly higher in the ET-Kyoto solution with ulinastatin (MK)/PFC preservation solution compared with the UW/PFC preservation solution in the porcine model and that the advantages of MK solution are trypsin inhibition and less collagenase inhibition. In this study, we compared ulinastatin with another trypsin inhibitor, Pefabloc, in preservation solution for islet isolation. Islet yield before purification was higher in the MK/PFC group compared with the ET-Kyoto with Pefabloc (PK)/PFC group. The stimulation index was higher for the MK/PFC group than for the PK/PFC group. These data suggest that ET-Kyoto with ulinastatin was the better combination for pancreas preservation than ET-Kyoto with Pefabloc. Based on these data, we now use ET-Kyoto solution with ulinastatin for clinical islet transplantation.

引用

页码：541 / 547

页数：7