High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection

被引：16

作者：

Mark, Laszlo ^{[1
]}

Patonai, Zoltan ^{[1
]}

Vaczy, Alexandra ^{[1
]}

Lorand, Tamas ^{[1
]}

Marcsik, Antonia ^{[2
]}

机构：

[1] Univ Pecs, Inst Med Chem & Biochem, Fac Med, H-7624 Pecs, Hungary

[2] Univ Szeged, Dept Anthropol, H-6722 Szeged, Hungary

来源：

JOURNAL OF ARCHAEOLOGICAL SCIENCE | 2010年 / 37卷 / 02期

基金：

美国国家科学基金会;

关键词：

Bone; MALDI TOF MS; MTC; Mycobacterium tubeculosis; Mycolic acid; Paleopathology; MYCOBACTERIUM-TUBERCULOSIS; BIOSYNTHESIS; DNA;

D O I：

10.1016/j.jas.2009.09.041

中图分类号：

Q98 [人类学];

学科分类号：

030303 ;

摘要：

The cell wall of mycobacteria includes an unusual outer membrane of extremely low permeability. This cell envelope consists of a characteristic cell wall skeleton, a mycoloyl arabinogalactan peptidoglycan complex, and related hydrophobic components that contribute to the cell surface properties. In this study 1400-year-old mycolic acids as unique tuberculosis biomarkers have been extracted and identified for the first time by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) and Fourier transform infrared spectroscopy (FT-IR). The data suggest that the MALDI TOF MS has potential as a rapid and reproducible technique for the detection and identification of ancient mycobacterial infections. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：302 / 305

页数：4

共 24 条

[1] The biosynthesis of mycolic acids by Mycobacteria:: current and alternative hypotheses [J].

Asselineau, C ;

Asselineau, J ;

Lanéelle, G ;

Lanéelle, MA .

PROGRESS IN LIPID RESEARCH, 2002, 41 (06) :501-523

[2] Pathogeny of archaic mycobacteria at the emergence of urban life in Egypt (3400 BC) [J].

Crubézy, E ;

Legal, L ;

Fabas, G ;

Dabernat, H ;

Ludes, B .

INFECTION GENETICS AND EVOLUTION, 2006, 6 (01) :13-21

[3]

Donoghue HD, 1998, LETT APPL MICROBIOL, V27, P265

[4] Mycolic acids and ancient DNA confirm an osteological diagnosis of tuberculosis [J].

Gernaey, AM ;

Minnikin, DE ;

Copley, MS ;

Dixon, RA ;

Middleton, JC ;

Roberts, CA .

TUBERCULOSIS, 2001, 81 (04) :259-265

[5]

Gross J.H., 2004, MASS SPECTROMETRY

[6]

Haas CJ, 2000, AM J PHYS ANTHROPOL, V113, P293, DOI 10.1002/1096-8644(200011)113:3<293::AID-AJPA2>3.0.CO

[7]

2-6

[8]

HAAS CJ, 1999, TUBERCULOSIS PAST PR, P385

[9] Detection and Molecular Characterization of 9000-Year-Old Mycobacterium tuberculosis from a Neolithic Settlement in the Eastern Mediterranean [J].

Hershkovitz, Israel ;

Donoghue, Helen D. ;

Minnikin, David E. ;

Besra, Gurdyal S. ;

Lee, Oona Y-C. ;

Gernaey, Angela M. ;

Galili, Ehud ;

Eshed, Vered ;

Greenblatt, Charles L. ;

Lemma, Eshetu ;

Bar-Gal, Gila Kahila ;

Spigelman, Mark .

PLOS ONE, 2008, 3 (10)

[10] Fatty acid biosynthesis in Mycobacterium tuberculosis:: Lateral gene transfer, adaptive evolution, and gene duplication [J].

Kinsella, RJ ;

Fitzpatrick, DA ;

Creevey, CJ ;

McInerney, JO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (18) :10320-10325

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