Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

被引：0

作者：

Zakashansky, Julia A. ^{[1
]}

Imamura, Amanda H. ^{[2
]}

Salgado, Darwin F., II ^{[3
]}

Romero Mercieca, Heather C. ^{[3
]}

Aguas, Raphael F. L. ^{[3
]}

Lao, Angelou M. ^{[3
]}

Pariser, Joseph ^{[3
]}

Arroyo-Curras, Netzahualcoyotl ^{[4
,5
,6
]}

Khine, Michelle ^{[3
]}

机构：

[1] Univ Calif Irvine, Mat Sci & Engn, Irvine, CA 92697 USA

[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil

[3] Univ Calif Irvine, Biomed Engn, Irvine, CA 92697 USA

[4] Johns Hopkins Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Whiting Sch Engn, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA

[6] Johns Hopkins Univ, Inst Nanobiotechnol, Baltimore, MD 21218 USA

来源：

ANALYTICAL METHODS | 2021年 / 13卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

ELECTROCHEMICAL DNA; APTAMERS; CORONAVIRUS; SPECIMENS;

D O I：

10.1039/d1ay00041a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using the children's toy, Shrinky-Dink (c), we present an aptamer-based electrochemical (E-AB) assay that recognizes the spike protein of SARS-CoV-2 in saliva for viral infection detection. The low-cost electrodes are implementable at population scale and demonstrate detection down to 1 ag mL(-1) of the S1 subunit of the spike protein.

引用

页码：874 / 883

页数：10

共 53 条

[1] RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein [J].

Ahn, Dae-Gyun ;

Jeon, Il-Ji ;

Kim, Jung Dong ;

Song, Min-Sun ;

Han, Seung-Ryul ;

Lee, Seong-Wook ;

Jung, Hyungil ;

Oh, Jong-Won .

ANALYST, 2009, 134 (09) :1896-1901

[2] Rapid, Ultrasensitive, and Quantitative Detection of SARS-CoV-2 Using Antisense Oligonucleotides Directed Electrochemical Biosensor Chip [J].

Alafeef, Maha ;

Dighe, Ketan ;

Moitra, Parikshit ;

Pan, Dipanjan .

ACS NANO, 2020, 14 (12) :17028-17045

[3]

Alejandra G.C., 2020, MOL BIOL+

[4] Presymptomatic SARS-CoV-2 Infections and Transmission in a Skilled Nursing Facility [J].

Arons, M. M. ;

Hatfield, K. M. ;

Reddy, S. C. ;

Kimball, A. ;

James, A. ;

Jacobs, J. R. ;

Taylor, J. ;

Spicer, K. ;

Bardossy, A. C. ;

Oakley, L. P. ;

Tanwar, S. ;

Dyal, J. W. ;

Harney, J. ;

Chisty, Z. ;

Bell, J. M. ;

Methner, M. ;

Paul, P. ;

Carlson, C. M. ;

McLaughlin, H. P. ;

Thornburg, N. ;

Tong, S. ;

Tamin, A. ;

Tao, Y. ;

Uehara, A. ;

Harcourt, J. ;

Clark, S. ;

Brostrom-Smith, C. ;

Page, L. C. ;

Kay, M. ;

Lewis, J. ;

Montgomery, P. ;

Stone, N. D. ;

Clark, T. A. ;

Honein, M. A. ;

Duchin, J. S. ;

Jernigan, J. A. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 382 (22) :2081-2090

[5] From the beaker to the body: translational challenges for electrochemical, aptamer-based sensors [J].

Arroyo-Curras, Netzahualcoyotl ;

Dauphin-Ducharme, Philippe ;

Scida, Karen ;

Chavez, Jorge L. .

ANALYTICAL METHODS, 2020, 12 (10) :1288-1310

[6] Presumed Asymptomatic Carrier Transmission of COVID-19 [J].

Bai, Yan ;

Yao, Lingsheng ;

Wei, Tao ;

Tian, Fei ;

Jin, Dong-Yan ;

Chen, Lijuan ;

Wang, Meiyun .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2020, 323 (14) :1406-1407

[7] Occurrence and transmission potential of asymptomatic and presymptomatic SARS-CoV-2 infections: A living systematic review and meta-analysis [J].

Buitrago-Garcia, Diana ;

Egli-Gany, Dianne ;

Counotte, Michel J. ;

Hossmann, Stefanie ;

Imeri, Hira ;

Ipekci, Aziz Mert ;

Salanti, Georgia ;

Low, Nicola .

PLOS MEDICINE, 2020, 17 (09)

[8]

CDC,, COR DIS 2019 COVID 1

[9] Solution-processed wrinkled electrodes enable the development of stretchable electrochemical biosensors [J].

Chan, Yuting ;

Skreta, Marta ;

McPhee, Hannah ;

Saha, Sudip ;

Deus, Ryan ;

Soleymani, Leyla .

ANALYST, 2019, 144 (01) :172-179

[10] Shrinky-Dink microfluidics: 3D polystyrene chips [J].

Chen, Chi-Shuo ;

Breslauer, David N. ;

Luna, Jesus I. ;

Grimes, Anthony ;

Chin, Wei-Chun ;

Leeb, Luke P. ;

Khine, Michelle .

LAB ON A CHIP, 2008, 8 (04) :622-624

← 1 2 3 4 5 6 →