Protective effect on enamel demineralization of a CPP-ACP paste: an AFM in vitro study

Objectives: The aim of the present in vitro study was the evaluation of a CPP-ACP paste (Tooth Mousse) on preventing dental erosion produced by a soft drink (Coca Cola), using Atomic Force Microscopy (AIM). Methods: Thirty extracted human central incisors free of caries were selected and divided in a treatment and a control half. The treatment halves were divided in three groups-group 1: demineralization with soft drink (4 intervals of 2 min); group 2: demineralization with soft drink (4 intervals of 2 min) plus Tooth Mousse; group 3: intact enamel plus Tooth Mousse. In groups 2 and 3 Tooth Mousse was applied for 3 min at 0, 8, 24 and 36 h. The surface of each specimen was imaged by AFM and R-rms values were registered. Results: Among treatment specimens of groups 1 and 2, a statistically significant difference (P < 0.01) in R-rms values was registered: treatment of the specimens with CPP-ACP paste had a protective effect on enamel demineralization. In group 3 no statistically significant difference was registered between exposed and not exposed halves of the specimens. Conclusions: The use of a CPP-ACP paste had a protective effect on enamel dernineralization in an in vitro model. (C) 2009 Elsevier Ltd. All rights reserved.