Multilocus sequence typing for the analysis of clonality among Candida albicans strains from a neonatal intensive care unit

被引：12

作者：

Song, Eun Song ^{[1
]}

Shin, Jong Hee ^{[2
]}

Jang, Hee-Chang ^{[3
]}

Choi, Min Ji ^{[2
]}

Kim, Soo Hyun ^{[2
]}

Bougnoux, Marie-Elisabeth ^{[4
,5
,6
]}

d'Enfert, Christophe ^{[4
,5
]}

Choi, Young Youn ^{[1
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Dept Paediat, Kwangju 501757, South Korea

[2] Chonnam Natl Univ, Sch Med, Dept Lab Med, Kwangju 501757, South Korea

[3] Chonnam Natl Univ, Sch Med, Dept Internal Med, Kwangju 501757, South Korea

[4] Inst Pasteur, Unite Biol & Pathogen Fong, Paris, France

[5] INRA USC2019, Paris, France

[6] Univ Paris 05, Fac Med, Hop Necker Enfants Malad, Lab Parasitol Mycol,Serv Microbiol, Paris, France

来源：

MEDICAL MYCOLOGY | 2014年 / 52卷 / 06期

关键词：

Candida albicans; neonatal intensive care unit; multilocus sequence typing; pulsed-field gel electrophoresis; genotyping; EPIDEMIOLOGY; INFECTIONS; INFANTS; DNA;

D O I：

10.1093/mmy/myu028

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nosocomial Candida albicans infections are a significant problem in neonatal intensive care units (NICUs). We investigated the clonality of C. albicans isolates recovered over an 8-year period from neonates at a NICU. We also validated multilocus sequence typing (MLST) compared with pulsed-field gel electrophoresis (PFGE) for the genotyping of C. albicans strains from the same NICU. A total of 43 clinical isolates (10 blood, 19 urine, and 14 other) were obtained from 43 neonates between 2005 and 2012. Clonal strains were defined as the isolation of two or more strains with identical or similar genotypes as determined with both MLST and PFGE. Using MLST, the 43 isolates yielded 25 diploid sequence types (DSTs) and 10 DSTs were shared by 28 isolates (65.1%). Among the 28 isolates sharing 10 DSTs, isolates from each of seven DSTs had the same or similar PFGE pattern. In addition, two sets of isolates that differed by MLST at only one locus had the same or similar PFGE pattern. Overall, when the MLST and PFGE results were combined, 22 isolates (51.2%) shared eight genotypes, suggesting clonal strains. Strains from each of seven genotypes (total, 19 isolates) were isolated among the 22 clonal strains within a 6-month period, whereas three strains of one genotype were obtained over a 3-year interval. Our findings suggest that horizontal transmission of C. albicans may occur more frequently than vertical transmission among NICU patients and that MLST appears to be a useful method for genotyping C. albicans strains isolated from NICU patients.

引用

页码：653 / 658

页数：6

共 10 条

[1]

[Anonymous], 2008, Approved standard M27-A. A National Committee for Clinical Laboratory Standards

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