Genetic Diversity of Cryptosporidium hominis in a Bangladeshi Community as Revealed by Whole-Genome Sequencing

被引：37

作者：

Gilchrist, Carol A. ^{[1
]}

Cotton, James A. ^{[2
]}

Burkey, Cecelia ^{[1
]}

Arju, Tuhinur ^{[3
]}

Gilmartin, Allissia ^{[1
]}

Lin, Ye ^{[4
]}

Ahmed, Emtiaz ^{[3
]}

Steiner, Kevin ^{[1
]}

Alam, Masud ^{[3
]}

Ahmed, Shahnawaz ^{[3
]}

Robinson, Guy ^{[5
,6
]}

Zaman, Sultan Uz ^{[3
]}

Kabir, Mamun ^{[3
]}

Sanders, Mandy ^{[2
]}

Chalmers, Rachel M. ^{[5
,6
]}

Ahmed, Tahmeed ^{[3
]}

Ma, Jennie Z. ^{[7
]}

Haque, Rashidul ^{[3
]}

Faruque, Abu S. G. ^{[3
]}

Berriman, Matthew ^{[2
]}

Petri, William A. ^{[1
]}

机构：

[1] Univ Virginia, Dept Med, Charlottesville, VA USA

[2] Wellcome Trust Sanger Inst, Cambridge, England

[3] Int Ctr Diarrhoeal Dis Res, Dhaka, Bangladesh

[4] Univ Virginia, Dept Stat, Charlottesville, VA USA

[5] Singleton Hosp, Publ Hlth Wales Microbiol, Cryptosporidium Reference Unit, Swansea, W Glam, Wales

[6] Swansea Univ, Sch Med, Singleton Pk, Swansea, W Glam, Wales

[7] Univ Virginia, Dept Publ Hlth Sci, Charlottesville, VA USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2018年 / 218卷 / 02期

基金：

英国惠康基金; 比尔及梅琳达.盖茨基金会; 美国国家卫生研究院;

关键词：

Cryptosporidium hominis; genome; genotype; gp60; parasite; SUBTYPES; PARVUM;

D O I：

10.1093/infdis/jiy121

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We studied the genetic diversity of Cryptosporidium hominis infections in slum-dwelling infants from Dhaka over a 2-year period. Cryptosporidium hominis infections were common during the monsoon, and were genetically diverse as measured by gp60 genotyping and whole-genome resequencing. Recombination in the parasite was evidenced by the decay of linkage disequilibrium in the genome over < 300 bp. Regions of the genome with high levels of polymorphism were also identified. Yet to be determined is if genomic diversity is responsible in part for the high rate of reinfection, seasonality, and varied clinical presentations of cryptosporidiosis in this population.

引用

页码：259 / 264

页数：6

共 15 条

[1] A new heterogeneous family of telomerically encoded Cryptosporidium proteins [J].

Bouzid, Maha ;

Hunter, Paul R. ;

McDonald, Vincent ;

Elwin, Kristin ;

Chalmers, Rachel M. ;

Tyler, Kevin M. .

EVOLUTIONARY APPLICATIONS, 2013, 6 (02) :207-217

[2] Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons [J].

Cama, Vitaliano A. ;

Ross, Jennifer M. ;

Crawford, Sara ;

Kawai, Vivian ;

Chavez-Valdez, Raul ;

Vargas, Daniel ;

Vivar, Aldo ;

Ticona, Eduardo ;

Navincopa, Marco ;

Williamson, John ;

Ortega, Ynes ;

Gilman, Robert H. ;

Bern, Caryn ;

Xiao, Lihua .

JOURNAL OF INFECTIOUS DISEASES, 2007, 196 (05) :684-691

[3] Suitability of loci for multiple-locus variable-number of tandem-repeats analysis of Cryptosporidium parvum for inter-laboratory surveillance and outbreak investigations [J].

Chalmers, Rachel M. ;

Robinson, Guy ;

Hotchkiss, Emily ;

Alexander, Claire ;

May, Sophie ;

Gilray, Janice ;

Connelly, Lisa ;

Hadfield, Stephen J. .

PARASITOLOGY, 2017, 144 (01) :37-47

[4] Cryptosporidiosis [J].

Chappell, CL ;

Okhuysen, PC .

CURRENT OPINION IN INFECTIOUS DISEASES, 2002, 15 (05) :523-527

[5] Comparative genomic analysis reveals occurrence of genetic recombination in virulent Cryptosporidium hominis subtypes and telomeric gene duplications in Cryptosporidium parvum [J].

Guo, Yaqiong ;

Tang, Kevin ;

Rowe, Lori A. ;

Li, Na ;

Roellig, Dawn M. ;

Knipe, Kristine ;

Frace, Michael ;

Yang, Chunfu ;

Feng, Yaoyu ;

Xiao, Lihua .

BMC GENOMICS, 2015, 16

[6] Generation of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples [J].

Hadfield, Stephen J. ;

Pachebat, Justin A. ;

Swain, Martin T. ;

Robinson, Guy ;

Cameron, Simon J. S. ;

Alexander, Jenna ;

Hegarty, Matthew J. ;

Elwin, Kristin ;

Chalmers, Rachel M. .

BMC GENOMICS, 2015, 16

[7] Natural History of Cryptosporidiosis in a Longitudinal Study of Slum-Dwelling Bangladeshi Children: Association with Severe Malnutrition [J].

Korpe, Poonum S. ;

Haque, Rashidul ;

Gilchrist, Carol ;

Valencia, Cristian ;

Niu, Feiyang ;

Lu, Miao ;

Ma, Jennie Z. ;

Petri, Sarah E. ;

Reichman, Daniel ;

Kabir, Mamun ;

Duggal, Priya ;

Petri, William A., Jr. .

PLOS NEGLECTED TROPICAL DISEASES, 2016, 10 (05)

[8] Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study [J].

Liu, Jie ;

Kabir, Furqan ;

Manneh, Jainaba ;

Lertsethtakarm, Paphavee ;

Begum, Sharmin ;

Gratz, Jean ;

Becker, Steve M. ;

Operario, Darwin J. ;

Taniuchi, Mami ;

Janaki, Lalitha ;

Platts-Mills, James A. ;

Haverstick, Doris M. ;

Kabir, Mamun ;

Sobuz, Shihab U. ;

Nakjarung, Kaewkanya ;

Sakpaisal, Pimmada ;

Silapong, Sasikom ;

Bodhidatta, Ladaporn ;

Qureshi, Shahida ;

Kalam, Adil ;

Saidi, Queen ;

Swai, Ndealilia ;

Mujaga, Buliga ;

Maro, Athanasia ;

Kwambana, Brenda ;

Dione, Michel ;

Antonio, Martin ;

Kibiki, Gibson ;

Mason, Carl J. ;

Haque, Rashidul ;

Iqbal, Najeeha ;

Zaidi, Anita K. M. ;

Houpt, Eric R. .

LANCET INFECTIOUS DISEASES, 2014, 14 (08) :716-724

[9] Entamoeba histolytica-associated diarrheal illness is negatively associated with the growth of preschool children:: evidence from a prospective study [J].

Mondal, Dinesh ;

Petri, William A., Jr. ;

Sack, R. Bradley ;

Kirkpatrick, Beth D. ;

Haque, Rashidul .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2006, 100 (11) :1032-1038

[10] OCCURRENCE AND SIGNIFICANCE OF CRYPTOSPORIDIUM INFECTION IN CALCUTTA [J].

PAL, S ;

BHATTACHARYA, SK ;

DAS, P ;

CHAUDHURI, P ;

DUTTA, P ;

DE, SP ;

SEN, D ;

SAHA, MR ;

NAIR, GB ;

PAL, SC .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1989, 83 (04) :520-521

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