Long Non-Coding RNAs Associated with Metabolic Traits in Human White Adipose Tissue

被引：45

作者：

Gao, Hui ^{[1
]}

Kerr, Alastair ^{[2
]}

Jiao, Hong ^{[1
]}

Hon, Chung-Chau ^{[3
]}

Ryden, Mikael ^{[2
]}

Dahlman, Ingrid ^{[2
]}

Arner, Peter ^{[2
]}

机构：

[1] Karolinska Inst, Dept Biosci & Nutr, Novum, Floor 7, S-14186 Stockholm, Sweden

[2] Karolinska Univ Hosp, Karolinska Inst, Dept Med, C2-94, S-14186 Stockholm, Sweden

[3] RIKEN Ctr Life Sci Technol, Div Genom Technol, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan

来源：

EBIOMEDICINE | 2018年 / 30卷

基金：

瑞典研究理事会;

关键词：

White adipose tissue; Adipocytes; Long non-coding RNAs; Metabolic traits; Lipolysis; Adiponectin; INSULIN-RESISTANCE; OBESITY; ADIPOGENESIS; LIPOLYSIS; LNCRNA; FAT;

D O I：

10.1016/j.ebiom.2018.03.010

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed in obesity and/or the insulin resistant state. Two lncRNAs herein termed adipocyte-specific metabolic related lncRNAs, ASMER-1 and ASMER-2 were enriched in adipocytes and regulated by both obesity and insulin resistance. Knockdown of either ASMER-1 or ASMER-2 by antisense oligonucleotides in in vitro differentiated human adipocytes revealed that both genes regulated adipogenesis, lipid mobilization and adiponectin secretion. The observed effects could be attributed to crosstalk between ASMERs and genes within the master regulatory pathways for adipocyte function including PPARG and INSR. Altogether, our data demonstrate that lncRNAs are modulators of the metabolic and secretory functions in human fat cells and provide an emerging link between WAT and common metabolic conditions. (C) 2018 The Authors. Published by Elsevier B.V.

引用

页码：248 / 260

页数：13

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