EFFECT OF NITRATE DEPLETION ON LIPID ACCUMULATION BY THE MARINE MICROALGA Nannochloropsis oculata

被引：0

作者：

Alves da Silva, Jose William ^{[1
]}

Araujo, Glacio Souza ^{[2
]}

da Cruz Coelho, Anderson Alan ^{[3
]}

Cavalcante Bezerra, Joao Henrique ^{[4
]}

Mascena Lopes, Dilliani Naiane ^{[4
]}

Lobo Farias, Wladimir Ronald ^{[4
]}

机构：

[1] Inst Fed Educ Ciencia & Tecnol Ceara Limoeiro Do, Rua Estevao Remigio,1145 Ctr, BR-62930000 Limoeiro Do Norte, CE, Brazil

[2] Inst Fed Educ Ciencia & Tecnol Ceara Aracati, BR-62800000 Aracati, CE, Brazil

[3] Inst Fed Educ Ciencia & Tecnol Ceara Morada Nova, BR-62900000 Morada Nova, CE, Brazil

[4] Univ Fed Ceara, BR-60455900 Fortaleza, Ceara, Brazil

来源：

BOLETIM DO INSTITUTO DE PESCA | 2015年 / 41卷

关键词：

biomass; lipid content; cells yield; CHLORELLA-VULGARIS; CULTIVATION; PROPERTY; GROWTH;

D O I：

暂无

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Nannochloropsis oculata is a marine algae with high lipid content, being a promising species for biofuel production. It is also used as food in aquaculture for larval fish and crustaceans due to its high nutritional value and compatible size with the larvae feeding. This study evaluated the lipid content in the biomass of N. oculata under nitrate depletion. The cultivation was carried out in 8 L bottles with three replications, at salinity 27, illuminance of 60 mu E cm(-2) s(-1) and temperature 28 degrees C. The levels of sodium nitrate used were 15; 30; 45; 60 and 75 mg L-1, while maintaining constant the levels of all other nutrients of the Guillard f/2 medium. The growth curve was monitored every two days by spectrophotometry at 680 nm (DO680nm) and cell counting in a Neubauer chamber. For separation of the culture medium from the cells, we used chemical flocculation method, adding 2N NaOH. The higher lipid content in the biomass was obtained at the lowest level of nitrate in the culture medium.

引用

页码：811 / 816

页数：6

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