Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus

被引:9
作者
Kong, Hee Jeong [1 ]
Hong, Gyeong-Eun [1 ]
Kim, Woo-Jin [1 ]
Kim, Young-Ok [1 ]
Nam, Bo-Hye [1 ]
Lee, Chang Hoon [2 ]
Do, Jeong Wan [3 ]
Lee, Jeong-Ho [4 ]
Lee, Sang-Jun [1 ]
Kim, Kyung-Kil [1 ]
机构
[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Inst, Pusan 619705, South Korea
[2] Pusan Natl Univ, Res Grp Marine & Silver Biotechnol, Pusan 609735, South Korea
[3] Natl Fisheries Res & Dev Inst, Div Pathol, Pusan 619705, South Korea
[4] Natl Fisheries Res & Dev Inst, Genet & Breeding Res Ctr, Geoje 656842, Gyeongnam, South Korea
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2009年 / 153卷 / 03期
关键词
Eukaryotic translation initiation factor 5A; Hypusine-containing protein; Olive flounder; Paralichthys olivaceus; Viral hemorrhagic septicemia virus; GC-7; INITIATION-FACTOR; 5A; HEMORRHAGIC SEPTICEMIA VIRUS; AMINO-ACID; POSTTRANSLATIONAL SYNTHESIS; DEOXYHYPUSINE SYNTHASE; MARINE FISH; IDENTIFICATION; PROLIFERATION; LOCALIZATION; TEMPERATURE;
D O I
10.1016/j.cbpb.2009.03.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eukaryotic translation initiation factor 5A (eIF5A) is the only protein in eukaryotic cells that contains the unusual amino acid hypusine (N(epsilon)-(4-amino-2(R)-hydroxybutyl)-lysine). We isolated a 1385-bp eIF5A cDNA containing an open reading frame (ORF) of 468 bp, which encodes a protein of 155 amino acids with a conserved hypusine modification site, from the olive flounder Paralichthys olivaceus. Pairwise alignments revealed that flounder eIF5A had a high sequence identity with those of other known species including mammals. Real-time RT-PCR analysis showed the expression of eIF5A mRNA was constitutively detected in various tissues of healthy flounder. In HINAE cells or flounder kidney infected with the viral hemorrhagic septicemia virus (VHSV), the expression of eIF5A mRNA was slightly increased before cells showed cytopathic effects and then decreased when cells showed cytopathic effects. Treatment of N-guanyl-1,7-diaminoheptane (GC-7), a potent inhibitor of eIF5A hypusination, inhibited the expression of VHSV G protein in a dose-dependent manner suggesting a potential role for eIF5A and its hypusination in viral protein expression. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:281 / 287
页数:7
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