Co-expression of heat shock transcription factors 1 and 2 in rat retinal ganglion cells

被引：16

作者：

Kwong, Jacky M. K.

Lalezary, Maziar

Nguyen, Jessica K.

Yang, Christine

Khattar, Anuj

Piri, Natik

Mareninov, Sergey

Gordon, Lynn K.

Caprioli, Joseph

机构：

[1] Univ Calif Los Angeles, Dept Ophthalmol, Jules Stein Eye Inst, David Geffen Sch Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Neurosci, Los Angeles, CA 90095 USA

[3] Univ Calif San Diego, Sch Med, San Diego, CA 92103 USA

[4] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[7] Greater Los Angeles VA Healthcare Syst, Ophthalmol Sect, Los Angeles, CA USA

来源：

NEUROSCIENCE LETTERS | 2006年 / 405卷 / 03期

关键词：

heat shock protein; stress rat; heat shock factor; retina; ganglion cell;

D O I：

10.1016/j.neulet.2006.06.070

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Heat shock protein (HSP) plays an important role in the maintenance of neuronal survival during harmful conditions. Previously, we reported that metabolic stress induces HSP72 in retinal ganglion cells (RGCs) and protects against excitotoxicity, hypoxia and experimental glaucoma. To understand heat shock protein transcriptional mechanisms, we examined the cellular expression of heat shock factors 1 (HSF1) and 2 (HSF2) in the unstressed adult rat retina. Western blotting, immunohistochemistry and RT-PCR showed that mRNA and protein of HSF1 and HSF2 were present in the rat retina and predominantly expressed in RGC layer cells. Western blotting of dissociated RGC suspensions harvested with Thy-1 immuno-labeled magnetic beads confirmed that RGCs expressed HSF1, HSF2 and HSP72. Our findings suggest that both heat shock transcription factors I and 2 are linked to the heat shock response in retinal ganglion cells. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：191 / 195

页数：5

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