On the nature and timing of oxygen radical production following exposure of Arabidopsis thaliana leaves to uranium, cadmium or a combination of both stressors

被引：7

作者：

Horemans, N. ^{[1
]}

Vanhoudt, N. ^{[1
]}

Janssens, M. ^{[1
]}

Van Chaze, B. ^{[1
]}

Wannijn, J. ^{[1
]}

Van Hees, M. ^{[1
]}

Vandenhove, H. ^{[1
]}

机构：

[1] Belgian Nucl Res Ctr SCKCEN, Biosphere Impact Studies, Boeretang 200, B-2400 Mol, Belgium

来源：

RADIOPROTECTION | 2011年 / 46卷 / 06期

关键词：

D O I：

10.1051/radiopro/20116761s

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The toxicity and oxidative stress responses of 19-day old Arabidopsis seedlings induced by U (66 mu M) and Cd (20 mu M) alone or in a binary mixture set-up (equitoxic mixture) are studied in function of time. After 48h a significant decrease in root and shoot growth and a simultaneous increase in anthocyanin production was evident in all treated plants. Production of O-2(center dot-) or H2O2 was visualized by staining freshly harvested leaves with nitrobluetetrazolium or diaminobezidine, respectively. With this method production of O-2(center dot-) was only significantly measurable after 168 h treatment which coincides with a significant decrease in biomass production and probably also plant cell death. For Cd treated plants a significant increase in H2O2 production was measurable from 24h onwards. In contrast, a similar H2O2 production could not be measured in U or U + Cd treated plants. Both water and lipophilic soluble antioxidants significantly increased in U treated plants after 48 h. These high antioxidant levels might detoxify potential H2O2 produced in the U treated plants. In contrast for Cd treated plants only after 168h a significant increase in water soluble antioxidants was measured whereas no difference in the lipophilic fraction was visible.

引用

页码：S491 / S496

页数：6