DNA barcodes reveal microevolutionary signals in fire response trait in two legume genera

被引：9

作者：

Bello, Abubakar ^{[1
]}

Daru, Barnabas H. ^{[2
]}

Stirton, Charles H. ^{[1
]}

Chimphango, Samson B. M. ^{[1
]}

van der Bank, Michelle ^{[3
]}

Maurin, Olivier ^{[3
]}

Muasya, A. Muthama ^{[1
]}

机构：

[1] Univ Cape Town, Dept Biol Sci, Bolus Herbarium, Private Bag X3, ZA-7700 Rondebosch, South Africa

[2] Univ Pretoria, Dept Plant Sci, ZA-0028 Pretoria, South Africa

[3] Univ Johannesburg, Dept Bot & Plant Biotechnol, African Ctr DNA Barcoding, ZA-2006 Johannesburg, South Africa

来源：

AOB PLANTS | 2015年 / 7卷

基金：

新加坡国家研究基金会;

关键词：

Fabaceae; Otholobium; Psoralea; reseeders; resprouters; South Africa; CAPE FLORISTIC REGION; DIVERSITY; ECOLOGY; PLANTS; IDENTIFICATION; RESPROUTERS; SPECIATION; CHURCHILL; SEEDERS; ORIGINS;

D O I：

10.1093/aobpla/plv124

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Large-scale DNA barcoding provides a new technique for species identification and evaluation of relationships across various levels (populations and species) and may reveal fundamental processes in recently diverged species. Here, we analysed DNA sequence variation in the recently diverged legumes from the Psoraleeae (Fabaceae) occurring in the Cape Floristic Region (CFR) of southern Africa to test the utility of DNA barcodes in species identification and discrimination. We further explored the phylogenetic signal on fire response trait (reseeding and resprouting) at species and generic levels. We showed that Psoraleoid legumes of the CFR exhibit a barcoding gap yielding the combination of matK and rbcLa (matK + rbcLa) data set as a better barcode than single regions. We found a high score (100 %) of correct identification of individuals to their respective genera but a very low score (<50 %) in identifying them to species. We found a considerable match (54 %) between genetic species and morphologically delimited species. We also found that different lineages showed a weak but significant phylogenetic conservatism in their response to fire as reseeders or resprouters, with more clustering of resprouters than would be expected by chance. These novel microevolutionary patterns might be acting continuously over time to produce multi-scale regularities of biodiversity. This study provides the first insight into the DNA barcoding campaign of land plants in species identification and detection of the phylogenetic signal in recently diverged lineages of the CFR.

引用

页数：19

共 68 条

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