Ultrasensitive and Selective Colorimetric DNA Detection by Nicking Endonuclease Assisted Nanoparticle Amplification

被引:354
|
作者
Xu, Wei [1 ]
Xue, Xuejia [1 ]
Li, Tianhu [2 ]
Zeng, Huaqiang [1 ]
Liu, Xiaogang [1 ]
机构
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[2] Nanyang Technol Univ, Div Chem & Biol Chem, Singapore 637371, Singapore
关键词
colorimetric detection; DNA; DNA cleavage; nanostructures; nicking endonuclease; ENHANCED RAMAN-SPECTROSCOPY; SEQUENCE-SPECIFIC DETECTION; GOLD NANOPARTICLES; MOLECULAR BEACONS; HOMOGENEOUS DETECTION; SIGNAL AMPLIFICATION; OPTICAL-PROPERTIES; LIGHT-SCATTERING; SENSITIVE DNA; RNA DETECTION;
D O I
10.1002/anie.200901772
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
One-two punch: Single stranded DNA sequences can be detected by nicking endonuclease assisted nanoparticle amplification (see picture). The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides. Detection of DNA sequences with a single base mismatch or different lengths is also demonstrated. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:6849 / 6852
页数:4
相关论文
共 50 条
  • [31] Highly Selective and Sensitive Electrochemiluminescence Biosensor for p53 DNA Sequence Based on Nicking Endonuclease Assisted Target Recycling and Hyperbranched Rolling Circle Amplification
    Yang, Linlin
    Tao, Yingzhou
    Yue, Guiyin
    Li, Ruibao
    Qin, Bin
    Guo, Longhua
    Lin, Zhenyu
    Yang, Huang-Hao
    ANALYTICAL CHEMISTRY, 2016, 88 (10) : 5097 - 5103
  • [32] Label-Free Colorimetric Aptasensor Based on Nicking Enzyme Assisted Signal Amplification and DNAzyme Amplification for Highly Sensitive Detection of Protein
    Huang, Yong
    Chen, Jia
    Zhao, Shulin
    Shi, Ming
    Chen, Zhen-Feng
    Liang, Hong
    ANALYTICAL CHEMISTRY, 2013, 85 (09) : 4423 - 4430
  • [33] Ultraselective Homogeneous Electrochemical Biosensor for DNA Species Related to Oral Cancer Based on Nicking Endonuclease Assisted Target Recycling Amplification
    Tan, Yue
    Wei, Xiaofeng
    Zhao, Mengmeng
    Qiu, Bin
    Guo, Longhua
    Lin, Zhenyu
    Yang, Huang-Hao
    ANALYTICAL CHEMISTRY, 2015, 87 (18) : 9204 - 9208
  • [34] Cascade signal amplification for ultrasensitive electrochemical DNA detection
    Xu, Jin
    Wang, Qiong
    Xiang, Yun
    Yuan, Ruo
    Chai, Yaqin
    ANALYST, 2014, 139 (01) : 128 - 132
  • [35] Ultrasensitive and selective signal-on electrochemical DNA detection via exonuclease III catalysis and hybridization chain reaction amplification
    Ren, Wang
    Gao, Zhong Feng
    Li, Nian Bing
    Luo, Hong Qun
    BIOSENSORS & BIOELECTRONICS, 2015, 63 : 153 - 158
  • [36] Ultrasensitive colorimetric aptasensor for Hg2+ detection using Exo-III assisted target recycling amplification and unmodified AuNPs as indicators
    Memon, Abdul Ghaffar
    Xing, Yunpeng
    Zhou, Xiaohong
    Wang, Ruoyu
    Liu, Lanhua
    Zeng, Siyu
    He, Miao
    Ma, Mei
    JOURNAL OF HAZARDOUS MATERIALS, 2020, 384
  • [37] Nicking enzyme-free strand displacement amplification-assisted CRISPR-Cas-based colorimetric detection of prostate-specific antigen in serum samples
    Wang, Wanhe
    Liu, Jianhua
    Wu, Li-An
    Ko, Chung-Nga
    Wang, Xueliang
    Lin, Chuankai
    Liu, Jingqi
    Ling, Liansheng
    Wang, Jing
    ANALYTICA CHIMICA ACTA, 2022, 1195
  • [38] Ultrasensitive electrochemical detection of microRNA with star trigon structure and endonuclease mediated signal amplification
    Miao, Peng
    Wang, Bidou
    Yu, Zhiqiang
    Zhao, Jing
    Tang, Yuguo
    BIOSENSORS & BIOELECTRONICS, 2015, 63 : 365 - 370
  • [39] Fabrication of a Highly Sensitive Aptasensor for Potassium with a Nicking Endonuclease-Assisted Signal Amplification Strategy
    Zhu, Xiaoli
    Zhao, Jing
    Wu, Yao
    Shen, Zhongming
    Li, Genxi
    ANALYTICAL CHEMISTRY, 2011, 83 (11) : 4085 - 4089
  • [40] Abasic Site-Assisted Inhibition of Nicking Endonuclease Activity for the Sensitive Determination of Uracil DNA Glycosylase
    Ahn, Jun Ki
    Lee, Chang Yeol
    Park, Ki Soo
    Park, Hyun Gyu
    BIOTECHNOLOGY JOURNAL, 2018, 13 (04)