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Ultrasensitive and Selective Colorimetric DNA Detection by Nicking Endonuclease Assisted Nanoparticle Amplification
被引:354
|作者:
Xu, Wei
[1
]
Xue, Xuejia
[1
]
Li, Tianhu
[2
]
Zeng, Huaqiang
[1
]
Liu, Xiaogang
[1
]
机构:
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[2] Nanyang Technol Univ, Div Chem & Biol Chem, Singapore 637371, Singapore
关键词:
colorimetric detection;
DNA;
DNA cleavage;
nanostructures;
nicking endonuclease;
ENHANCED RAMAN-SPECTROSCOPY;
SEQUENCE-SPECIFIC DETECTION;
GOLD NANOPARTICLES;
MOLECULAR BEACONS;
HOMOGENEOUS DETECTION;
SIGNAL AMPLIFICATION;
OPTICAL-PROPERTIES;
LIGHT-SCATTERING;
SENSITIVE DNA;
RNA DETECTION;
D O I:
10.1002/anie.200901772
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
One-two punch: Single stranded DNA sequences can be detected by nicking endonuclease assisted nanoparticle amplification (see picture). The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides. Detection of DNA sequences with a single base mismatch or different lengths is also demonstrated. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
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页码:6849 / 6852
页数:4
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