Ultrasensitive and Selective Colorimetric DNA Detection by Nicking Endonuclease Assisted Nanoparticle Amplification

被引：354

作者：

Xu, Wei ^{[1
]}

Xue, Xuejia ^{[1
]}

Li, Tianhu ^{[2
]}

Zeng, Huaqiang ^{[1
]}

Liu, Xiaogang ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Nanyang Technol Univ, Div Chem & Biol Chem, Singapore 637371, Singapore

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 37期

关键词：

colorimetric detection; DNA; DNA cleavage; nanostructures; nicking endonuclease; ENHANCED RAMAN-SPECTROSCOPY; SEQUENCE-SPECIFIC DETECTION; GOLD NANOPARTICLES; MOLECULAR BEACONS; HOMOGENEOUS DETECTION; SIGNAL AMPLIFICATION; OPTICAL-PROPERTIES; LIGHT-SCATTERING; SENSITIVE DNA; RNA DETECTION;

D O I：

10.1002/anie.200901772

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

One-two punch: Single stranded DNA sequences can be detected by nicking endonuclease assisted nanoparticle amplification (see picture). The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides. Detection of DNA sequences with a single base mismatch or different lengths is also demonstrated. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6849 / 6852

页数：4

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