Application of magnetic nanoparticles in full-automated chemiluminescent enzyme immunoassay

被引：20

作者：

Xie, Xiaomao ^{[1
]}

Ohnishi, Noriyuki ^{[1
]}

Takahashi, Yuki ^{[1
]}

Kondo, Akihiko ^{[2
]}

机构：

[1] Magnabeat Inc, Chiba 2908551, Japan

[2] Kobe Univ, Dept Sci & Chem Engn, Fac Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2009年 / 321卷 / 10期

关键词：

Magnetic nanoparticle; Full-automation; Chemiluminescent enzyme immunoassay; Thyroid-stimulating hormone; Light emission; PARTICLES; SEPARATION;

D O I：

10.1016/j.jmmm.2009.02.115

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The magnetic nanoparticles (MNPs) Therma-Max (TM) were used as a carrier to develop an automated sandwich chemiluminescent enzyme immunoassay (CLEIA) to detect thyroid-stimulating hormone (TSH) in a sensitive and specific way. The Therma-Max (TM) particles allow for automation because, unlike magnetic microspheres, they are completely dispersed in aqueous solution and allow for accurate automatic handling. Signal intensities detected with MNPs were 8-fold higher than those found with conventional micron-sized magnetic particles. A reproducibility study suggests that these particles allow for a stable detection method, as the coefficient of variation(CV) is less than 6% (n = 10). (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1686 / 1688

页数：3

共 17 条

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