1,25(OH)2D3 attenuates TGF-β1/β2-induced increased migration and invasion via inhibiting epithelial-mesenchymal transition in colon cancer cells

被引：41
作者：
Chen, Shanwen [1 ]
Zhu, Jing [1 ]
Zuo, Shuai [1 ]
Ma, Ju [1 ]
Zhang, Junling [1 ]
Chen, Guowei [1 ]
Wang, Xin [1 ]
Pan, Yisheng [1 ]
Liu, Yucun [1 ]
Wang, Pengyuan [1 ]
机构：
[1] Peking Univ, Hosp 1, Div Gen Surg, Beijing 100034, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 468卷 / 1-2期
关键词：
1,25(OH)2D3; Colon cancer; EMT; VDR; SW-480; HT-29; VITAMIN-D ANALOG; N-CADHERIN; 1-ALPHA; 25-DIHYDROXYVITAMIN D-3; TGF-BETA; EXPRESSION; ADHESION; PROGRESSION; MART-10; SWITCH;
D O I：
10.1016/j.bbrc.2015.10.146
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been reported to inhibit proliferation and migration of multiple types of cancer cells. However, the mechanism underlying its anti-metastasis effect is not fully illustrated. In this study, the effect of 1,25(OH)2D3 on TGF-beta 1/beta 2-induced epithelial mesenchymal transition (EMT) is tested in colon cancer cells. The results suggest that 1,25(OH)2D3 inhibited TGF-beta 1/beta 2-induced increased invasion and migration of in SW-480 and HT-29 cells. 1,25(OH)2D3 also inhibited the cadherin switch in SW-480 and HT-29 cells. TGF-beta 1/beta 2-induced increased expression of EMT-related transcription factors was also inhibited by 1,25(OH)2D3. 1,25(OH)2D3 also inhibited the secretion of MMP-2 and MMP-9 and increased expression of F-actin induced by TGF-beta 1/beta 2 in SW-480 cells. Taken together, this study suggests that the suppression of EMT might be one of the mechanisms underlying the anti-metastasis effect of 1,25(OH)2D3 in colon cancer cells. (C) 2015 Elsevier Inc. All rights reserved.
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页码：130 / 135
页数：6
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